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�й�ҩѧ��־ 2010, Vol. 45 Issue (13) :1032-1034    DOI:
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HPLC-ELSD�ⶨ̫�ױ�ĸ���߲���ĸ�б�ĸ���ĺ���
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LIU Jing1, WANG Shu2, XIN Gui-zhong1, LI Hui-jun1, LI Ping1*
1. Key Laboratory of Modern Chinese Medicines, Ministry of Education, Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China; 2.West China School of Pharmacy, Sichuan University, Chengdu 610041, China

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ժҪ Ŀ�� �ⶨ����ĸ�µĻ�Դֲ��̫�ױ�ĸ��Fritillaria taipaiensis P. Y. Li�����߲���ĸ[Fritillaria unibracteata Hsiao et K. C. Hsia var.wabuensis (S. Y. Tang et S. C. Yue) Z. D. Liu, S. Wang et S. C. Chen]�۾��б�ĸ���ĺ��������� ����HPLC-ELSD����ɫ����ΪAgilent Hypersil BDS-C18(4.0 mm��250 mm��5 ��m)��������Ϊ����-ˮ-���Ұ��ܼ�ϵͳ���ݶ�ϴ�ѣ�����Ϊ1.0 mL��min-1��������ɢ��������⡣��� ��ĸ����40.75��815.00 ��g��mL-1�ڣ������������������������������Թ�ϵ��r=0.999 4������ĸ������������Ϊ98.7%��RSDΪ2.5% (n= 6)��̫�ױ�ĸ�б�ĸ������Ϊ0.017 3%��0.037 6%���߲���ĸ�б�ĸ������Ϊ0.023 3%��0.052 8%������ ����̫�ױ�ĸ���߲���ĸ��Ʒ�б�ĸ���ĺ����������������Ĵ���ĸ������Դֲ����Ʒ��
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Abstract�� OBJECTIVE To determine the content of peimisine in Fritillaria taipaiensis and Fritillaria unibracteata, the novel resource of fritillariae cirrhosae bulbus. METHODS A high performance liquid chromatography coupled with evaporative light scattering detector was employed, and peimisine was separated and quantified upon an Agilent Hypersil BDS-C18 column (4.0 mm��250 mm��5 ��m) using acetonitrile-water-diethylamine as the mobile phase in a gradient program at the flow rate of 1.0 mL��min-1. RESULTS Within the test range of 40.75-815.00 ��g��mL-1, the logarithm of peak area of peimisine versus the logarithm of nominal mass showed good correlation (r=0.999 4), and the mean recovery of analyte was 98.7% with RSD of 2.5% (n=6). The obtained content varied from 0.017 3% to 0.037 6% in Fritillaria taipaiensis and from 0.023 3% to 0.052 8% in Fritillaria unibracteata, respectively. CONCLUSION Fritillaria taipaiensis and Fritillaria unibracteata contained higher content of peimisine than fritillariae cirrhosae bulbus.
Keywords�� fritillariae cirrhosae bulbus,   Fritillaria taipaiensis,   Fritillaria unibracteata,   peimisine,   HPLC-ELSD     
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.HPLC-ELSD�ⶨ̫�ױ�ĸ���߲���ĸ�б�ĸ���ĺ���[J]  �й�ҩѧ��־, 2010,V45(13): 1032-1034
.Determination of Peimisine in Fritillaria taipaiensis and Fritillaria unibracteata by HPLC-ELSD[J]  Chinese Pharmaceutical Journal, 2010,V45(13): 1032-1034
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