中国药学杂志
    
           首页  |  期刊介绍  |  编 委 会  |  投稿指南  |  期刊订阅  |  广告服务  |  会议信息  |  联系我们  | 
�й�ҩѧ��־ 2010, Vol. 45 Issue (16) :1250-1254    DOI:
�� �� ����Ŀ¼ | ����Ŀ¼ | ������� | �߼����� << | >>
�ؾ�ʯ�᳤���������֬������Ʊ���������ʲⶨ�����о�
�ƺ���1����ΰ2���Ż�2��֣Ф��3��÷�˹�2*
1. ����ʡ��ҽҩ�о�Ժ����ɳ 410006��2. ����ҽѧ��ѧԺ����ҩ���о��������� 100850��3. �Ϻ����ȷ�ҩҵ���޹�˾��ҵ�����ģ��Ϻ� 201203
HUANG He-song1�� GONG Wei2�� ZHANG Hui2�� ZHENG Xiao-li3�� MEI Xing-guo2*
1. Hunan Academy of Chinese Medicine�� Changsha 410006�� China; 2.Institute of Pharmacology and Toxicology�� Academy of Military Medical Sciences�� Beijing 100850, China; 3.Shanghai Asiapioneer Pharmaceuticals�� Shanghai 201203, China

Download: PDF (1170KB)   HTML (0KB)   Export: BibTeX or EndNote (RIS)      Supporting Info
ժҪ Ŀ�� �Ʊ��ؾ�ʯ�᳤�������vinorelbine tartrate�� VRT������֬���壬����֬������VRT�����Ͱ����ʵIJⶨ���������� pH�ݶȷ��Ʊ�VRT����֬���壬HPLC�ⶨ֬������VRT�ĺ�����΢�����ķ�����֬����������ҩ�������Ͼ����������͡�ϴ���ܼ������ߡ�ϴ����������ض�VRT����ҩϴ�ѵ�Ӱ�졣��� VRT����֬�����ƽ������Ϊ��94.6��1.6��nm������Ϊ��1.83��0.03��mg��mL-1��ѡ���Ͼ�������G-25�Ʊ�΢�����������4 cm����PBS����ҺΪϴ���ܼ����ݶ�ϴ�����������֬����������ҩ�����3��VRT����֬����İ����ʷֱ�Ϊ��95.75��1.42��%����91.12��1.21��%����94.10��2.03��%��ϴ�ѻ����ʷֱ�Ϊ��98.3��2.42��%����93.5��1.83��%����96.6��1.65��%������ VRT֬������Ʊ������ȶ�����ҩ���󣬰����ʸߡ�������������ʲⶨ�����򵥡����١�׼ȷ����ʵ���ΪVRT����ע��������֬�������Ƽ��Ŀ����ṩ�о�������
Service
�ѱ����Ƽ�������
�����ҵ����
�������ù�����
Email Alert
RSS
�����������
�ؼ����� �ؾ�ʯ�᳤�����   ����֬����   ΢������     
Abstract�� OBJECTIVE To prepare vinorelbine tartrate��VRT�� thermo-sensitive liposomes and establish methods for determining its VRT content and entrapment efficiency. METHODS VRT thermo-sensitive liposomes were prepared by pH-gradients method. VRT contents were determined by HPLC. Liposomes and free drug were separated by mini-column centrifugation. The influential factors of VRT elution including sephadex type�� elution solution�� and column height and elution volume were investigated. RESULTS Mean particle size of VRT thermo-sensitive liposomes was ��94.6��1.6��nm. VRT content was ��1.83��0.03�� mg��mL-1. The entrapment efficiencies of three batches of VRT thermo-sensitive liposomes were ��95.75��1.42��%�� ��91.12��1.21��% and ��94.10��2.03��%�� and the elution recoveries were ��98.3��2.42��%�� ��93.5��1.83��% and ��96.6��1.65��%. Sephadex G-25 was chosen to prepare mini-column. Elution solution were PBS�� 4 cm of column height was optimal�� and one of gradients-elution-volumes was ascertained. CONCLUSION The preparation technology of VRT liposomes was stable�� drug loading and entrapping capacity of VRT liposomes were well. The methods for determination of VRT content and entrapment efficiency were simple�� rapid and accurate. These studies can provide bases for further development of intravenous injection VRT thermo-sensitive liposomes.
Keywords�� vinorelbine tartrate,   thermo-sensitive liposome,   mini-column centrifugation,   entrapment efficiency
     
�ո�����: 2012-01-01;
���ñ���:   
.�ؾ�ʯ�᳤���������֬������Ʊ���������ʲⶨ�����о�[J]  �й�ҩѧ��־, 2010,V45(16): 1250-1254
.Preparation and Entrapment Efficiency of Vinorelbine Tartrate Thermo-sensitive Liposomes[J]  Chinese Pharmaceutical Journal, 2010,V45(16): 1250-1254
��
û�б��IJο�����
Copyright 2010 by �й�ҩѧ��־