人参皂甘Rg1对AngⅡ所致心肌细胞肥大的抑制作用

王英婷 黄燮南 王风安

中国药学杂志 ›› 2010, Vol. 45 ›› Issue (18) : 1380-1383.

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中国药学杂志 ›› 2010, Vol. 45 ›› Issue (18) : 1380-1383.
论 著

人参皂甘Rg1对AngⅡ所致心肌细胞肥大的抑制作用

  • 王英婷1,黄燮南1,王风安2
作者信息 +

Inhibition Effects of Ginsenoside Rg1 on Cardiomyocyte Hypertrophy Induced by Angiotensin Ⅱ

  • WANG Ying-ting1, HUANG Xie-nan1, WANG Feng-an2
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文章历史 +

摘要

目的 探讨人参皂苷Rg1 (ginsenoside Rg1, Rg1) 对血管紧张素Ⅱ(angiotensin Ⅱ, AngⅡ)所致心肌细胞肥大的影响,并初探其分子机制。方法 在培养的新生大鼠心肌细胞中加入AngⅡ 0.1 μmol· L-1,HE染色观测细胞形态学变化,并以心肌细胞直径﹑蛋白含量和心房利钠因子(atrial natriuretic factor,ANF) mRNA的表达为肥大指标。为分析药物的作用机制,用Fura-2/AM负载心肌细胞检测细胞内游离钙浓度[Ca2+]i;并检测钙调神经磷酸酶(calcineurin, CaN)mRNA的表达变化。ANF 和CaN mRNA的表达采用Real-time PCR测定。结果 AngⅡ 0.1 μmol·L-1使心肌细胞直径明显增大,蛋白质含量明显增加,并使 ANF mRNA的表达显著升高。加入Rg1 15.6、31.2和62.4 μmol·L-1使AngⅡ所增大的心肌细胞直径分别缩短13%、33%、43% (P<0.01);并使心肌细胞蛋白含量分别减少10%、14%和19%(P<0.01),Rg1 62.4 μmol·L-1 还显著抑制AngⅡ所上调的ANF mRNA的表达。与此同时,上述浓度的Rg1还使AngⅡ所升高的 [Ca2+]i分别抑制了19.3%﹑28%和38.6%(P<0.01,n=6); Rg1 62.4 μmol·L-1还使AngⅡ所升高 CaN mRNA的表达明显降低。结论 Rg1可抑制AngⅡ诱导的心肌细胞肥大,该作用可能与其降低由AngⅡ所升高的心肌细胞[Ca2+]i,并由此而抑制Ca2+-CaN信号通路有关。

Abstract

OBJECTIVE To observe the effects of ginsenoside Rg1(Rg1)on cardio myocyte hypertrophy induced by angiotensin Ⅱ(AngⅡ), and to investigate its molecular mechanism. METHODS AngⅡ-induced neonatal rat cardiomyocyte hypertrophic responses and the antihypertrophic effects of Rg1 were assayed by measuring the cell diameter, protein content and the expression of atrial natriuretic factor(ANF) mRNA. For analyzing the antihypertrophic mechanism, the intracellular free Ca2+ concentration ([Ca2+]i) and the calcineurin(CaN) mRNA expression in cultured cardiomyocytes were measured by using Fura 2/AM as fluorescent indicator and by Real-time PCR, respectively. RESULTS AngⅡ 0.1 μmol·L-1 induced significantly the increases of the cell diameter, protein content and the expression of ANF mRNA in cultured cardiomyocytes. Ginsenoside Rg1 15.6, 31.2, 62.4 μmol·L-1 concentration-dependently inhibited the elevated cell diameter by 13%, 33% and 43% (P<0.01, n=3), and the elevated protein content by 10%, 14% and 19%(P<0.01, n=6), compared with AngⅡ-model group. The up regulated ANF mRNA expression induced by AngⅡ was significantly decreased by Rg1 62.4 μmol·L-1. The increase of AngⅡ-induced [Ca2+]i was inhibited by 19.3%, 28% and 38.6%, respectively, and 62.4 μmol·L-1 Rg1 remarkably decreased the expression of CaN mRNA. CONCLUSION Rg1 can inhibit the cardiomyocyte hypertrophy induced by AngⅡ, which may be related to its inhibitory effect on the elevated [Ca2+]i, resulting in inactivation of CaN transduction pathway.

关键词

血管紧张素Ⅱ / 人参皂苷Rg1 / 心肌肥大 / 钙调神经磷酸酶

Key words

angiotensin Ⅱ / ginsenoside Rg1 / cardic myocyte hypertrophy / calcineurin

引用本文

导出引用
王英婷 黄燮南 王风安. 人参皂甘Rg1对AngⅡ所致心肌细胞肥大的抑制作用[J]. 中国药学杂志, 2010, 45(18): 1380-1383
WNG Ying-ting;HUNG Xie-nn;WNG Feng-n. Inhibition Effects of Ginsenoside Rg1 on Cardiomyocyte Hypertrophy Induced by Angiotensin Ⅱ[J]. Chinese Pharmaceutical Journal, 2010, 45(18): 1380-1383

参考文献


[1] BERENJI K, DRAZNER M H, ROTHERMEL B A, et al. Dose load-induced ventricular hypertrophy progress to systolic heart failure?[J].Am J Physiol Heart Circ Physiol, 2005, 289(1):8-16.
[2] KRISHNAN P, VENTURA H O, UBER P A, et al. Treatment of hypertension for patients with diastolic dysfunction[J]. Curr Opin Cardiol, 2003, 18(4): 272-277.
[3] HARADA K, KOMURO I, SHIOJIMA I, et al. Pressure overload induces cardiac hypertrophy in angiotensin Ⅱ type 1A receptor knockout mice [J]. Circulation, 1998,97(19):1952-1959.
[4] VARAGIC J, FROHLICH E D. Local cardiac rennin-angiotensin system: hypertension and cardiac failure [J]. J Mol Cell Cardiol, 2002,34(11):1435-1442.
[5] YAMAZAKI T, KOMURO I, YAZAKI Y. Role of the rennin-angiotension system in cardiac hypertrophy[J].Am J Cardiol, 1999, 83(12A): 53-57.
[6] SERNERI G G, BODDI M, CECIONI I, et al.Cardiac angiotensinⅡ formation in the clinical course of heart failure and its relationship with left ventricular function[J]. Circ Res, 2001, 88(9):961-968.
[7] KUDOH S, AKAZAWA H, TAKANO H, et al. Stretch modulation of second messengers: effects on cardiomyocyte ion transport[J]. Prog Biophys Mol Biol, 2003, 82(1-3):57-66.
[8] DENG J, HUANG X N. Anti-cardiac hypertrophic effect of ginsenoside Rg1[J]. Chin J Clin Pharmacol Ther(中国临床药理学与治疗学), 2008, 13(3):271-275.
[9] E Z. Tissue Culture and Molecular Cytology Technology[M]. 2nd ed. Beijing: Beijing Publishing House, 1997:131-132.
[10] ADAMS J W, MIGITA D S, YU M K, et al. Prostaglandin F alpha stimulates hypertrophic growth of cultured neonatal rat ventricular myocytes [J].J Biol Chem, 1996, 271(2):1179-1186.
[11] XU S Y, BIAN R L, CHEN X. Pharmacological Experimental Methodology(药理实验方法学)[M]. 3rd ed. Beijing: People's Medical Publishing House, 2002:1026-1030.
[12] PAN D S, TIAN C, CHEN L Y. On the mechanisms of cardiac hypertrophy[J]. Prog Physiol Sci, 1999, 30(2):113-117.
[13] FU M G, TANG C S. Cellular signal transduction in cardiac hypertrophy[J]. Bas Med Sci Clin, 2000, 20(3):1-4.
[14] LAI J, JIN H,YAN R, et al. Prostaglandin F induced cardiac hypertrophy in vitro and cardiac growth in vivo[J]. Am J Physiol Heart Circul Physiol, 1996, 40(6):2197-2208.
[15] MOLKENTIN J D, LU J R, ANTOS C L, et al. A calcineurin-dependent transcriptional pathway for cardiac hypertrophy[J]. Cell, 1998, 93(2):215-228.
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