Abstract��
OBJECTIVE To observe the effects of ginsenoside Rg1(Rg1)on cardio myocyte hypertrophy induced by angiotensin ��(Ang��)�� and to investigate its molecular mechanism. METHODS Ang��-induced neonatal rat cardiomyocyte hypertrophic responses and the antihypertrophic effects of Rg1 were assayed by measuring the cell diameter�� protein content and the expression of atrial natriuretic factor(ANF) mRNA. For analyzing the antihypertrophic mechanism�� the intracellular free Ca2+ concentration ([Ca2+]i) and the calcineurin(CaN) mRNA expression in cultured cardiomyocytes were measured by using Fura 2/AM as fluorescent indicator and by Real-time PCR�� respectively. RESULTS Ang�� 0.1 ��mol��L-1 induced significantly the increases of the cell diameter�� protein content and the expression of ANF mRNA in cultured cardiomyocytes. Ginsenoside Rg1 15.6�� 31.2�� 62.4 ��mol��L-1 concentration-dependently inhibited the elevated cell diameter by 13%�� 33% and 43% (P<0.01�� n=3)�� and the elevated protein content by 10%�� 14% and 19%(P<0.01�� n=6)�� compared with Ang��-model group. The up regulated ANF mRNA expression induced by Ang�� was significantly decreased by Rg1 62.4 ��mol��L-1. The increase of Ang��-induced [Ca2+]i was inhibited by 19.3%�� 28% and 38.6%�� respectively�� and 62.4 ��mol��L-1 Rg1 remarkably decreased the expression of CaN mRNA. CONCLUSION Rg1 can inhibit the cardiomyocyte hypertrophy induced by Ang�� which may be related to its inhibitory effect on the elevated [Ca2+]i�� resulting in inactivation of CaN transduction pathway.
.Inhibition Effects of Ginsenoside Rg1 on Cardiomyocyte Hypertrophy Induced by Angiotensin ��[J] Chinese Pharmaceutical Journal, 2010,V45(18): 1380-1383
2010, Vol. 45 
