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�й�ҩѧ��־ 2010, Vol. 45 Issue (18) :1380-1383    DOI:
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�˲����Rg1��Ang�������ļ�ϸ���ʴ����������
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1.����ҽѧԺҩ������ң����� ���� 563003��2.����������ҽԺ������ ���� 454001
WANG Ying-ting1�� HUANG Xie-nan1�� WANG Feng-an2
1. Department of Pharmacology�� Zunyi Medical College�� Zunyi 563003��China;2. Jiaozuo People��s Hospital�� Jiaozuo 454001�� China

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ժҪ Ŀ�� ̽���˲�����Rg1 (ginsenoside Rg1�� Rg1) ��Ѫ�ܽ����آ�(angiotensin �� Ang��)�����ļ�ϸ���ʴ��Ӱ�죬����̽����ӻ��ơ����� �����������������ļ�ϸ���м���Ang�� 0.1����mol�� L-1��HEȾɫ�۲�ϸ����̬ѧ�仯�������ļ�ϸ��ֱ���p���׺������ķ��������ӣ�atrial natriuretic factor��ANF�� mRNA�ı��Ϊ�ʴ�ָ�ꡣΪ����ҩ������û��ƣ���Fura-2/AM�����ļ�ϸ�����ϸ���������Ũ��[Ca2+]i�������Ƶ�������ø��calcineurin�� CaN��mRNA�ı��仯��ANF ��CaN mRNA�ı�����Real-time PCR�ⶨ����� Ang�� 0.1 ��mol��L-1ʹ�ļ�ϸ��ֱ���������󣬵����ʺ����������ӣ���ʹ ANF mRNA�ı���������ߡ�����Rg1 15.6��31.2��62.4 ��mol��L-1ʹAng����������ļ�ϸ��ֱ���ֱ�����13%��33%��43% (P<0.01)����ʹ�ļ�ϸ�����׺����ֱ����10%��14%��19%(P<0.01)��Rg1 62.4 ��mol��L-1 ����������Ang�����ϵ���ANF mRNA�ı����ͬʱ������Ũ�ȵ�Rg1��ʹAng�������ߵ� [Ca2+]i�ֱ�������19.3%�p28%��38.6%(P<0.01��n=6)�� Rg1 62.4 ��mol��L-1��ʹAng�������� CaN mRNA�ı�����Խ��͡����� Rg1������Ang���յ����ļ�ϸ���ʴ󣬸����ÿ������併����Ang�������ߵ��ļ�ϸ��[Ca2+]i�����ɴ˶�����Ca2+-CaN�ź�ͨ·�йء�
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Abstract�� OBJECTIVE To observe the effects of ginsenoside Rg1(Rg1)on cardio myocyte hypertrophy induced by angiotensin ��(Ang��)�� and to investigate its molecular mechanism. METHODS Ang��-induced neonatal rat cardiomyocyte hypertrophic responses and the antihypertrophic effects of Rg1 were assayed by measuring the cell diameter�� protein content and the expression of atrial natriuretic factor(ANF) mRNA. For analyzing the antihypertrophic mechanism�� the intracellular free Ca2+ concentration ([Ca2+]i) and the calcineurin(CaN) mRNA expression in cultured cardiomyocytes were measured by using Fura 2/AM as fluorescent indicator and by Real-time PCR�� respectively. RESULTS Ang�� 0.1 ��mol��L-1 induced significantly the increases of the cell diameter�� protein content and the expression of ANF mRNA in cultured cardiomyocytes. Ginsenoside Rg1 15.6�� 31.2�� 62.4 ��mol��L-1 concentration-dependently inhibited the elevated cell diameter by 13%�� 33% and 43% (P<0.01�� n=3)�� and the elevated protein content by 10%�� 14% and 19%(P<0.01�� n=6)�� compared with Ang��-model group. The up regulated ANF mRNA expression induced by Ang�� was significantly decreased by Rg1 62.4 ��mol��L-1. The increase of Ang��-induced [Ca2+]i was inhibited by 19.3%�� 28% and 38.6%�� respectively�� and 62.4 ��mol��L-1 Rg1 remarkably decreased the expression of CaN mRNA. CONCLUSION Rg1 can inhibit the cardiomyocyte hypertrophy induced by Ang�� which may be related to its inhibitory effect on the elevated [Ca2+]i�� resulting in inactivation of CaN transduction pathway.
Keywords�� angiotensin ��,   ginsenoside Rg1,   cardic myocyte hypertrophy,   calcineurin     
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.�˲����Rg1��Ang�������ļ�ϸ���ʴ����������[J]  �й�ҩѧ��־, 2010,V45(18): 1380-1383
.Inhibition Effects of Ginsenoside Rg1 on Cardiomyocyte Hypertrophy Induced by Angiotensin ��[J]  Chinese Pharmaceutical Journal, 2010,V45(18): 1380-1383
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