LIANG Jian-mou1��2�� QIU Feng2�� WANG Nai-li2�� YANG Xiao-juan1�� TU Peng-fei1�� JIANG Yong1*
1.State Key Laboratory of Natural and Biomimetic Drugs�� School of Pharmaceutical Sciences�� Peking University Health Science Center��Beijing 100191�� China; 2. Department of Natural Products Chemistry�� School of Traditional Chinese Medicine�� Shenyang Pharmaceutical University�� Shenyang 110016�� China
Abstract��
OBJECTIVE To investigate the factors influencing the stability of 3��6��-disinapoyl sucrose�� and to clarify the suitable storage method for its further research and development. METHODS UV and HPLC methods were used to detect the stability of 3��6��-disinapoyl sucrose in different solvents�� temperatures�� pH values�� and illuminations. HPLC-DAD-MS was used to analyze the transformed products of 3��6��-disinapoyl sucrose after illuminating under the sunshine and the UV lamp of 365 nm. RESULTS There was good correlation between the results of UV and HPLC methods. The results showed that the stability of 3��6��-disinapoyl sucrose was influenced by the solvent�� pH value�� temperature and illumination. The analysis result of the transformed products by UV and MS indicated that the isomers of cis and trans-double bond of the sinapoyl group were the main transformed products of 3��6��-disinapoyl sucrose after illumination. CONCLUSION UV method could be used for the rapid analysis of the stability of 3��6��-disinapoyl sucrose. During the separation�� purification and analytical process of this compound�� it prefer to a neutral or an acid alcoholic solvent in a low temperature�� and to keep away from the light.
2010, Vol. 45 
