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�й�ҩѧ��־ 2010, Vol. 45 Issue (21) :1623-1627    DOI:
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����ͪ��ApoEȱ��С��������ƽ����ϸ������Ƥϸ����֬�ر��ͷ��ڵ�Ӱ��
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1.Ϋ��ҽѧԺ������������ң�ɽ�� Ϋ�� 261053��2.���пƼ���ѧͬ��ҽѧԺ������������ң��人 430030
ZHANG Dai-juan1�� LIU Tong-mei1��LIU Jiang-yue1�� CUI Xiao-dong1�� GUO Jun-tang1�� WANG Jan-ying1��YE Du-yun2*
1.Department of Pathophysiology�� Weifang Medical College�� Weifang 261053, China��2. Pathophysiology Department of Tongji Medical College of Huazhong University of Science and Technology�� Wuhan 430030, China

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ժҪ Ŀ�� �۲��Ѫ������ҩ����ͪ��ApoE(-/-)С������������Ӳ�����䣬Ѫ֬���߿�����Ҫϸ����֬�أ�visfatin������Ӱ�졣���� ȡ��ͨС����������������ƽ����ϸ������Ƥϸ����ԭ��������ʵ���5�飺�հ׶����飬IL-1���飬����ͪ�߼����飬����ͪ�ͼ����飬�շ���͡�����顣�ռ�����ϸ������Һ��Ӧ��ELISA�������ϸ������֬�ء�ȡ8ֻ8������ͨС�������������飻ȡ24ֻ8��������ApoE(-/-)С������ֳ����飺ģ����(n=8)��ÿ��������ˮ��θ������ͪ������(n=8)im��ҩ��10 mg��kg-1��d-1���շ���͡������(n=8)ig��ҩ��10 mg��kg-1��d-1������ʵ��С������ԡ�����������ʳ��������12�ܡ�ȡѪ�����֬�ء�Ѫ֬�ȣ���ȡ������������Ƭ��Ⱦɫ���羵�¹۲�����������Ӳ���߿���ƽ����ϸ������Ƥϸ����̬ѧ�仯�������黯Ⱦɫ���۲�߿�����֬�صķֲ��������� ����ͪ�߼�����Ѫ��ƽ����ϸ������Ƥϸ������Һ����֬�����Ե���IL-1����(P<0.01)�������������飻�շ���͡���������Ƹı䣬������֮���в���(P<0.01)��������ʵ��������ͪ��������֬�ؼ��١�Ѫ֬���ͣ��շ���͡�������Ѫ֬���ͣ�AS�����γɽԼ��٣��ڰ߿�����Ƥϸ����ƽ����ϸ����ʧ���ᡣ���� ��Ѫ������ҩ����ͪ�ܼ���ApoE(-/-)С�������������߿���γɣ�����ͨ��������֬�����ɣ�������Ѫ֬���������á�
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Abstract�� OBJECTIVE To observe the effects of 3��4-dihydroxyacetophenone(DHAP ) on blood lipids and plaque in ApoE (-/-) aorta atherosclerosis and the effects of DHAP on visfatin expression of vascular smooth muscle cells and endothelial cells. METHODS Normal thoracic aortas were obtained from eight week-old mice�� and the VSMC and EC were cultured. The cells were divided into five groups�� A. control group; B. IL-1�� group; C.high dose of DHAP group; D. low dose of DHAP group; E. Pravastatin group. The changes of visfatin were analyzed by ELISA. There were 8 eight-week-old normal mice in the normal control group. 24 eight-week-old male ApoE (-/-) mice were randomly divided into three groups�� model group (n=8)�� treated with normal saline; DHAP treatment group (n=8) treated with DHAP 10 mg��kg-1��d-1 intramuscularly; pravastatin treatment group (n=8) by gastric tube perfusion with pravastatin 10 mg��kg-1��d-1. All mice were fed with Western diet (21% fat�� 0.15% cholesterol) for 12 weeks. Then all mice were sacrificed by the end of the experiment��Their blood was collected for determining the concentration of blood lipids and visfatin. The morphology and composition of atherosclerotic plaques in aortic roots were examined in tissue sections��Four sections were stained with HE or immunohistochemical staining �� respectively. After computer image scanning�� the ratio of plaque area to luminal area were determined. RESULTS In the group treated with high dose of DHAP and pravastatin�� the supernatant visfatin of vascular smooth muscle cells endothelial cells was significantly lower than that of IL-1�� group (P<0.01)�� but higher than that of the control group. Similar changes were found in pravastatin group. There were differences between DHAP and pravastatin group (P<0.01). In vitro�� the plasma visfatin and lipids decreased in DHAP treatment group�� only plasma lipids decreased in pravastatin treatment group. AS lesion formation was decreased in these two groups. The injury of VSMC and EC was relieved at plaques. CONCLUSION The anti-inflammtory traditional drug����DHAP could slow down the atherosclerosis plaque formation in ApoE (-/-) mice�� possibly through inhibiting the expression of visfatin and reducing blood lipids .
Keywords�� anti-inflammatory tradational drug,   3,   4-dihydroxyacetophenone,   visfatin,   atherosclerosis     
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.����ͪ��ApoEȱ��С��������ƽ����ϸ������Ƥϸ����֬�ر��ͷ��ڵ�Ӱ��[J]  �й�ҩѧ��־, 2010,V45(21): 1623-1627
.Effects of Dihydroxyacetophenone on the Expression of Visfatin in ApoE(-/-) Mice and the Secretion of Visfatin in Vascular Smooth Muscle Cell�� Endothelial Cell[J]  Chinese Pharmaceutical Journal, 2010,V45(21): 1623-1627
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