ZHANG Dai-juan1�� LIU Tong-mei1��LIU Jiang-yue1�� CUI Xiao-dong1�� GUO Jun-tang1�� WANG Jan-ying1��YE Du-yun2*
1.Department of Pathophysiology�� Weifang Medical College�� Weifang 261053, China��2. Pathophysiology Department of Tongji Medical College of Huazhong University of Science and Technology�� Wuhan 430030, China
Abstract��
OBJECTIVE To observe the effects of 3��4-dihydroxyacetophenone(DHAP ) on blood lipids and plaque in ApoE (-/-) aorta atherosclerosis and the effects of DHAP on visfatin expression of vascular smooth muscle cells and endothelial cells. METHODS Normal thoracic aortas were obtained from eight week-old mice�� and the VSMC and EC were cultured. The cells were divided into five groups�� A. control group; B. IL-1�� group; C.high dose of DHAP group; D. low dose of DHAP group; E. Pravastatin group. The changes of visfatin were analyzed by ELISA. There were 8 eight-week-old normal mice in the normal control group. 24 eight-week-old male ApoE (-/-) mice were randomly divided into three groups�� model group (n=8)�� treated with normal saline; DHAP treatment group (n=8) treated with DHAP 10 mg��kg-1��d-1 intramuscularly; pravastatin treatment group (n=8) by gastric tube perfusion with pravastatin 10 mg��kg-1��d-1. All mice were fed with Western diet (21% fat�� 0.15% cholesterol) for 12 weeks. Then all mice were sacrificed by the end of the experiment��Their blood was collected for determining the concentration of blood lipids and visfatin. The morphology and composition of atherosclerotic plaques in aortic roots were examined in tissue sections��Four sections were stained with HE or immunohistochemical staining �� respectively. After computer image scanning�� the ratio of plaque area to luminal area were determined. RESULTS In the group treated with high dose of DHAP and pravastatin�� the supernatant visfatin of vascular smooth muscle cells endothelial cells was significantly lower than that of IL-1�� group (P<0.01)�� but higher than that of the control group. Similar changes were found in pravastatin group. There were differences between DHAP and pravastatin group (P<0.01). In vitro�� the plasma visfatin and lipids decreased in DHAP treatment group�� only plasma lipids decreased in pravastatin treatment group. AS lesion formation was decreased in these two groups. The injury of VSMC and EC was relieved at plaques. CONCLUSION The anti-inflammtory traditional drug����DHAP could slow down the atherosclerosis plaque formation in ApoE (-/-) mice�� possibly through inhibiting the expression of visfatin and reducing blood lipids .
.Effects of Dihydroxyacetophenone on the Expression of Visfatin in ApoE(-/-) Mice and the Secretion of Visfatin in Vascular Smooth Muscle Cell�� Endothelial Cell[J] Chinese Pharmaceutical Journal, 2010,V45(21): 1623-1627