Abstract��
OBJECTIVE To develop a RP-HPLC method for the simultaneous determination of venlafaxine (VL and its active metabolite O-desmethylvenlafaxine (ODV in serum.METHODS VL and ODV in serum were extracted by ether and were back-extracted from the organic layer into hydrochloric acid.The chromatographic separation was performed on the VP-ODS column using the mixture of methanol-water-butyl-Amine-glacial acetic acid (54��60��1.5��1.2 as the mobile phase.The detection wave length was at 229 nm.RESULTS The linearity was obtained in the range of 31.25-500 ��g��L-1 with the recoveries of 92.3%-125.7% and 109.9%-121.8% for ODV and VL respectively. The intraday RSDs were in the range of 5.52%-7.70% and 1.76%-9.46%�� and the inter-day RSDs were in the range of 4.59%-7.70% and 1.96%-9.59% for ODV and VL�� respectively.CONCLUSION This method was found to be accurate and reliable. It can be applied to the pharmacokinetic study and therapeutic drug monitoring of ODV and VL.
2011, Vol. 46 
