Abstract��
OBJECTIVE Effects of several environmental factors such as pH�� temperature and light were investigated on mycelial growth�� polysaccharide content of mycelia and sclerotial development of Polyporus umbellatus. METHODS Classical methods of cultivating fungi with petri dish using modified PDA agar medium were conducted to evaluate growth conditions of P. umbellatus. Colony diameter was measured to estimate the growth rate of P. umbellatus. The polysaccharide content of mycelia of P. umbellatus was detected with Phenol-Sulphate colorimetry. RESULTS The initial pH of 8 was the most suitable pH value for growth of P. umbellatus�� meanwhile sclerotia of P. umbellatus was successfully induced at the same conditions. Mycelia grew best at 25 �� and light inhibited growth of P. umbellatus. Among the mycelia of P. umbellatus being cultured by modified PDA medium with different pH values�� polysaccharide content of Group pH 8�� 9 and 10 was highest�� followed by that of Group pH 5 and 6. Polysaccharide content of Group pH 4 and 7 was the lowest. Among the mycelia of P. umbellatus being cultured by modified PDA medium at different temperatures�� polysaccharide content of Group 5 �� was the highest�� followed by that of Group 15 �棬 10 �� and 25 ��. Polysaccharide content of Group 30 �� was the lowest. CONCLUSION Environmental factors could greatly affect mycelial growth and sclerotial formation of P. umbellatus. This study might provide preparatory knowledge for sclerotial formation under artificial cultivation.
2011, Vol. 46 
