PAN Qun1��2�� QIN Yong-ping1*�� NAN Feng1�� XIANG Jin1�� LIANG Mao-zhi1��YU Qin1
1.Department of Clinical Pharmacology�� GCP center West China Hospital�� Sichuan University�� Chengdu 610041��China�� 2. West China School of Pharmacy�� Sichuan University�� Chengdu 610041��China
Abstract��
OBJECTIVE To establish a RP-HPLC method for the determination of cefozopran in human plasma and urine.METHODS The assay was conducted on a HPLC-UV system consisted of a Welch Materials XB C18 column (4.6 mm��150 mm�� 5 ��m . The mobile phase for plasma and urine were acetonitrile-0.01 mol��L-1 sodium acetate-triethylamine(6��94��0.001�� pH adjusted to 3.52 with acetic acid and acetonitrile-0.01 mol��L-1 sodium acetate-triethylamine(3��97��0.001�� pH adjusted to 3.55 with acetic acid�� respectively. Detection was performed at 235 nm. Plasma sample was first treated with acetonitrile for precipitation of proteins. The supernatant was then back washed by dichloromethane. The internal standard was Floxuridine. Urine was simply diluted with purified water and quantified by external reference method. RESULTS For plasma�� the calibration curve was linear in the range from 0.15 to 307.2 mg��L-1 �� the limit of quantitation was 0.15 mg��L-1�� the recovery of pretreatment was 86.9%-108.4%�� the inter- and intra-day RSDs were less than 2.4% and 5.4%�� respectively. For urine�� the calibration curve was linear in the range from 4.69 to 4 800 mg��L-1�� the limit of quantitation was 4.69 mg��L-1�� the inter- and intra-day RSDs were less than 3.0% and 5.9%�� respectively.CONCLUSION The methods is simple�� rapid�� sensitive and accurate for the determination of cefozopran in human plasma and urine.
2011, Vol. 46 
