ZHOU Xiao-bing1�� GAO Kai1�� SHEN Lian-zhong1�� ZHAO Ai-zhi2�� KANG Yu2�� WANG Gang2�� WU Xiao-bing2�� WANG Jun-zhi1�� LI Bo1*
1.National Center for Safety Evaluation of Drugs�� National Institute for the Control of Pharmaceutical and Biological Products�� Beijing 100083�� China��2. AGTC Gene Technology Company Ltd.�� Beijing 100176�� China
Abstract��
OBJECTIVE To investigate the effect of intramuscular rAAV2/rTNFR��Fc gene transfer on collagen induced arthritis in rats. METHODS An experimental arthritis model was induced by intradermal injection of �� type collagen. All rats were randomly divided into five groups (10 animals per group including normal�� model�� vector�� dexamethasone control group and rAAV2/rTNFR��Fc-treated group. Arthritis was evaluated by arthritis index�� paw swelling�� radiographic and histological examination. The serum TNF-�� and TNFR level were determined. RESULTS Compared with the model and vector control group�� anti-inflammatory effect was observed in the rAAV2/rTNFR��Fc -treated group as the arthritis index and paw swelling had significant reduced (P<0.05. The radiography examination showed that no significant destruction of bony were found while histopathologic analysis displayed relatively synoviocytes hyperplasia and neutrophil infiltration reduction in group treated with rAAV2/rTNFR��Fc. Furthermore�� following administration of rAAV2/rTNFR��Fc�� serum TNF-�� level significant decreased while TNFR level greatly increased (P<0.05. CONCLUSION The results indicate that rAAV2/rTNFR��Fc has anti-arthritic properties in the experimental model.
.Anti-Inflammatory Effect of Intramuscular rAAV2/rTNFR��Fc Gene Transfer on Collagen Induced Arthritis in Rats[J] Chinese Pharmaceutical Journal, 2011,V46(7): 507-511
2011, Vol. 46 
