1. Beijing Friendship Hospital�� Capital Medical University�� Beijing 100050�� China�� 2. Department of Biology and Center for Chinese Medicine�� Hong Kong University of Science and Technology�� Hong Kong
Abstract��
OBJECTIVE To establish the HPLC fingerprints for assessing the quality of Fructus Aurantii from different habitats��and to determine the contents of naringin�� neohesperidin and synephrine. METHODS HPLC fingerprinting and content determination methods were applied to evaluate 10 batches of Fructus Aurantii from different habitats.The samples were separated by Alltima C18 column(4.6 mm��250 mm��5 ��m using acetonitrile-0.1�� phosphoric acid and 0.1�� SDS water solution as mobile phase with gradient elution.The flow rate was 1.0 mL��min-1. The detection wavelengths were 224 nm for fingerprinting�� 283 nm for content determination of naringin and neohesperidin�� and 224 nm for content determination of synephrine�� respectively.RESULTS HPLC fingerprint of Fructus Aurantii was established with good separation and repeatability��which could be used for quality assessment of Fructus Aurantii.Six common peaks were defined in characteristic fingerprints�� and similarity evaluation system was applied to evaluate the fingerprints of the 10 batches of Fructus Aurantii. The contents of naringin�� neohesperidin and synephrine in Fructus Aurantii from different habitats were obviously different from each other. CONCLUSION The method is sensitive�� repeatable and accurate�� which can be used for the quality control of Fructus Aurantii.The contents of naringin�� neohesperidin and synephrine vary significantly in Fructus Aurantii from different habitats�� which suggests that more attention should be paid to the clinical use of Fructus Aurantii.
.Analysis of HPLC Fingerprints of Fructus Aurantii from Different Habitats and Contents of Naringin�� Neohesperidin and Synephrine[J] Chinese Pharmaceutical Journal, 2011,V46(12): 955-959
2011, Vol. 46 