摘要
目的 研究生药厚朴提取物（MOCE）及其主要成分厚朴酚与和厚朴酚对于常见口腔致病菌生长和黏附的影响。方法 选取2种致龋菌和2种牙周病致病菌作为实验菌株，用杯碟法和溶液稀释法分别考察其对4种口腔致病菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC），并考察杀菌速率；另建立黏附实验模型，考察药物对于致龋菌的黏附抑制作用，及其对已形成黏附的脱附作用。结果 MOCE对于4种实验菌株的MIC、MBC值均小于100 μg·mL-1，而且具有突出的速效杀菌特点：1 mg·mL-1MOCE 30 s内速效杀菌达99%以上，在MBC浓度下仅5 min 即已表现出明显杀菌作用（>90%）；对于致龋菌的黏附抑制，最小有效浓度为6.25~25 μg·mL-1（随菌种的不同而不同），且该浓度下对已形成的黏附具有脱附作用。结论 MOCE对4种口腔致病菌的生长具有较强抑制作用，速效杀菌，并对致龋菌的黏附具有抑制形成和促进瓦解的双效作用，在口腔用药和保健领域具有重要价值。
Abstract
OBJECTIVE To evaluate the antimicrobial properties of Magnoliae officinalis（MOCE） Cortex extract and its main components， magnolol and honokiol against two cariogenic bacteria strains and two periodontal pathogens. METHODS The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against each strain were determined by both cylinder-plate and broth-microdilution METHODS， and bactericidal rate， bacteria adhesion inhibition and bacteria desorption rates were measured by our experimental models. RESULTS The MIC and MBC of MOCE and its two main components against each strain were all less than 100 μg·mL-1， and the minimum effective concentrations for adhesion inhibition and desorption of two cariogenic bacteria were 6.25 and 25 μg·mL-1， respectively. Incubation of the bacteria with MOCE solution at 1 mg·mL-1 killed more than 99% of the test strains in 30 s. The extract at MBC against each strain also showed significant bacteriocidal effect in 5 min (>90%). CONCLUSION Our studies provided experimental evidence for further development and application of MOCE in the field of oral care.
关键词
厚朴 /
致龋菌 /
牙周病致病菌 /
厚朴酚 /
和厚朴酚
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Key words
Magnoliae officinalis Cortex /
cariogenic bacteria /
periodontal pathogens /
magnolol /
honokiol
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殷其蕾 刘勇 詹先王 韩南银 李金陆 杨圣辉.
厚朴提取物对于4种常见口腔致病菌生长和黏附的作用[J]. 中国药学杂志, 2011, 46(17): 1356-1361
YIN Qi-lei;LIU Yong;ZHN Xin-wng;HN Nn-yin;LI Jin-lu;YNG Sheng-hui.
Evaluation of the Antimicrobial Properties of Magnoliae officinalis Cortex Extract and Its Main Components Against Four Oral Pathogenic Bacteria[J]. Chinese Pharmaceutical Journal, 2011, 46(17): 1356-1361
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