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�й�ҩѧ��־ 2011, Vol. 46 Issue (20) :1605-1608    DOI:
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YIN Xiao-ying�� JIANG Yi-fan�� YANG Hua-sheng�� FU Jian-jiang�� LIU Hong-ning�� LUO Yong-ming*
Jiangxi University of Traditional Chinese Medicine College of Pharmacy�� Nanchang 330004�� China

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ժҪ Ŀ�� ������ЧҺ��ɫ�׷��ⶨ�޲��鶨ʱ����Ƭ��5�ַ��ἰ��ͪ��ɷֺ��������������� ����Hypersil C18��4.6 mm��200 mm��ɫ����������-10 mmol��L-1����炙���Һ��pH 4��������ڣ�Ϊ�����࣬ϴ�ѳ���0 min�� 10% ���棻8 min�� 15%���棻16 min�� 17%���棻40 min�� 38% ���档����1.0 mL��min-1����Ⲩ��Ϊ256 nm����� 2�ַ��ἰ3�ֻ�ͪ�ɷֵķ������Ũ�ȵ����Թ�ϵ���� (r>0.999 0) ������������Ϊ��94.3��1.80��%~��102.4��2.89��%������ �������򵥡�׼ȷ�������ԽϺ� �� �������޲��鶨ʱ����Ƭ���������Ŀ��ƺ����ۡ�
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Abstract�� OBJECTIVE To establish a method for determination of 5 caffeoylquinic acids and avonoids in Luobuma time-controlled Pulse tablet by HPLC. METHODS The HPLC method was performed on a Hypersil C18 column (4.6 mm��200 mm�� 5 ��m). The mobile phase was composed of acetonitril-10 mmol��L-1 ammonium acetate aqeous solution (pH 4.0�� adjusted with acetic acid solution) at a flow rate of 1 mL��min-1. The gradient elution procedure was as follows�� 0 min��10% acetonitril�� 8 min�� 15% acetonitril�� 16 min�� 17% acetonitril�� 40 min�� 38% acetonitril. The UV detection wavelength was 254 nm. RESULTS The calibration curves for the 2 caffeoylquinic acids and 3 flavonoids showed good linearity (r>0.999 0) in the range of the tested concentrations. The average recoveries of the 5 compounds were in the range of ��94.3��1.80��%-��102.4��2.89��%. CONCLUSION The method is simple�� accurate and reproducible�� and can be used to control and evaluate the quality of Luobuma time-controlled pulse tablet.
Keywords�� Apocynum venetum L.,   time-controlled pulsatile drug delivery system,   phenolic acids,   flavonoids,   determination     
�ո�����: 2011-11-11;
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.�޲��鶨ʱ����Ƭ��5�ַ��ἰ��ͪ��ɷֵĺ����ⶨ[J]  �й�ҩѧ��־, 2011,V46(20): 1605-1608
.Determination of 5 Flavonoids and Phenolic Acids in Luobuma Time-Controlled Pulse Tablets[J]  Chinese Pharmaceutical Journal, 2011,V46(20): 1605-1608
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[1] LIU X H�� ZHANG Y C�� LI J S�� et al. Simultaneous determination of four flavonoids in folium apocyni veneti by HPCE-DAD[J]. Chin Pharm J(�й�ҩѧ��־)�� 2010��45(6)��464-467.[2] MENG M J�� ZENG H S�� LI J�� et al. CZE Determination of five flavonoids i n Apocynum venetum from different regions [J]. Chin J Pharm Anal (ҩ�������־)��2010�� 30 (3)�� 405-408.[3] YIN X Y�� FU J J�� YANG H S�� et al. 2010 First International Conference on Cellular�� Molecular Biology�� Biophysics and Bioengineering��December 25-26��2010[C]. Qiqihar�� IEEE�� 2010��vol.3�� 285-288.[4] KAMATA K�� SEO S�� NAKAJIMA J. Constituents from leaves of Apocynum venetum L [J]. J Nat Med�� 2008�� 62(2)��160-163.
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