活血止痛胶囊中土鳖虫的分子鉴定研究

王云灵 濮存海 马毅敏 李红琴[.延边大学药学院 吉林 延吉 00;.南京中山制药有限公司 南京 00;.中国药科大学 南京 000;.南京医药产业

中国药学杂志 ›› 2011, Vol. 46 ›› Issue (20) : 1562-1565.

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中国药学杂志 ›› 2011, Vol. 46 ›› Issue (20) : 1562-1565.
论著

活血止痛胶囊中土鳖虫的分子鉴定研究

  • 王云灵1,濮存海2*,马毅敏3,李红琴4[1.延边大学药学院,吉林 延吉 133002;2.南京中山制药有限公司,南京 210046;3.中国药科大学,南京 210009;4.南京医药产业
作者信息 +

Molecular Identification of the Cockroach Eupolyphaga sinensis in a Compound Chinese Medicine, Huoxuezhitong Capsule

  • WANG Yun-ling1, PU Cun-hai2*, MA Yi-min3, Li Hong-qin4
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文章历史 +

摘要

目的 建立活血止痛胶囊中土鳖虫的一种分子鉴定方法方法 采用蛋白酶K法结合吸附柱法提取活血止痛胶囊中土鳖虫基因组DNA,使用蜚蠊目昆虫通用引物扩增其线粒体16S rRNA 片段并测序。结果 土鳖虫能扩增出475 bp左右的片段,本实验成功从自制活血止痛胶囊中扩增出该片段。结论方法灵敏度高,可靠性强,可以做为活血止痛胶囊中土鳖虫的一种鉴定方法

Abstract

OBJECTIVE To establish a molecular determination method of the cockroach Eupolyphaga sinensis in a compound Chinese medicine, Huoxuezhitong Capsule. METHODS The proteinase K conjugation adsorption column method was used to extract and purify genomic DNA. Using the universal primer of Blattaria, 16S rRNA was amplified and sequenced. RESULTS A fragment about 475 bp was obtained from Eupolyphaga sinensis. The self-made Huoxuezhitong capsule by different processing METHODS could be amplified successfully. Eupolyphaga sinensis and Huoxuezhitong capsule purchased from the market could not be amplified. CONCLUSION The method is reliable, and can be used for quality control of Huoxuezhitong capsule.

关键词

活血止痛胶囊 / 土鳖虫 / DNA提取 / 16S rRNA / 测序

Key words

Huoxuezhitong capsule / Eupolyphaga sinensis / DNA extraction / 16S rRNA / sequencing

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王云灵 濮存海 马毅敏 李红琴[.延边大学药学院 吉林 延吉 00;.南京中山制药有限公司 南京 00;.中国药科大学 南京 000;.南京医药产业. 活血止痛胶囊中土鳖虫的分子鉴定研究[J]. 中国药学杂志, 2011, 46(20): 1562-1565
WNG Yun-ling;PU Cun-hi;M Yi-min;Li Hong-qin. Molecular Identification of the Cockroach Eupolyphaga sinensis in a Compound Chinese Medicine, Huoxuezhitong Capsule[J]. Chinese Pharmaceutical Journal, 2011, 46(20): 1562-1565

参考文献


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