LIU Lua�� LI Linc�� MIN Junb�� WANG Jieb�� ZENG Yu-jiea�� WU Henga�� CHEN Shuanga�� CHU Zhong-huaa*
a.Department of Gastrointestinal surgery�� b. Department of Hepato-Biliary Surgery�� c.Department of Obstetrics and Gynecology�� Sun Yat-sen Memorial Hospital of Sun Yat-sen University�� Guangzhou 510120�� China
Abstract��
OBJECTIVE To investigate the immunological effects of different concentrations of sodium butyrate on dendritic cells (DC) differentiation in vitro. METHODS Human monocyte-derived DC were induced in the presence of cytokine recombined human granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4. The effects of different concentrations of sodium butyrate on DC were detected by FCM�� endocytic activity�� T cells stimulatory proliferation capacity�� and IL-10�� IL-12 and INF-�� production. RESULTS Sodium butyrate of 1.25 and 1.5 mmol��L-1 had much higher toxicity on DC compared with sodium butyrate below 1 mmol��L-1. All three concentrations of sodium butyrate below 1 mmol��L-1 could down-regulate the major histocompatibility complex (MHC) class II and costimulatory molecules of DC. Sodium butyrate of 1 mmol��L-1 had better suppression effects on mature markers of DC than other groups (0.5 and 0.75 mmol��L-1)�� although the endocytic activity of DC had no significant differences among the three groups (P>0.05). Sodium butyrate of 1.0 mmol��L-1 had the strongest capacity to induce a stage of T-cell anergy��1.53��1.04��and promote IL-10 production (3.29��0.21) compared with those of 0.5 and 0.75 mmol��L-1 sodium butyrate. CONCLUSION 1.0 mmol��L-1 is the most effective concentration of sodium butyrate for inducing immaturation state of dendritic cells.
.Comparison of the Immunological Effect of Sodium Butyrate of Different Concentrations on the Differentiation of Dendritic Cells[J] Chinese Pharmaceutical Journal, 2011,V46(24): 1874-1878
��
[1]
ONAI N�� MANZ M G. The STATs on dendritic cell development[J]. Immunity�� 2008�� 28(4)�� 490-492.[2] BARRATT-BOYES S M�� THOMSON A W. Dendritic cells�� tools and targets for transplant tolerance[J]. Am J Transplant�� 2005�� 5(12)�� 2807-2813.[3] NOURI-SHIRZAI M�� THOMSON A W. Dendritic cells as promoters of transplant tolerance[J]. Expert Opin Biol Ther�� 2006�� 6(4)�� 325-339.[4] HIKAWA H�� LOTZE M T�� ROSENBLUM W D. Induction of peripheral tolerance by local delivery of dendritic cell progenitors to cardiac allografts in a murine heterotopic heart transplantation model[J]. Gen Thorac Cardiovasc Surg�� 2007�� 55(8)��307-314.[5] FANG Y�� GENG L�� DONG S�� et al. Effects of cyclosporine A on murine bone marrow-derived dendritic cells[J].Chin Pham J(�й�ҩѧ��־)�� 2006�� 41(13)��983-986.[6] CAYO M A�� CAYO A K�� JARJOUR S M�� et al. Sodium butyrate activates Notch1 signaling�� reduces tumor markers�� and induces cell cycle arrest and apoptosis in pheochromocytoma[J]. Am J Transl Res�� 2009�� 1(2)��178-183.[7] LIU L�� MIN J�� WANG J�� et al. Study on the immunological function of sodium butyrate-induced immature human monocyte-derived dendritic cells[J]. Chin J Patho-Physiol (�й�����������־)�� 2007�� 23(6)��1199-1203.[8] GAGLIARDI M C�� TELONI R�� GIANNONI F�� et al. Mycobacterium bovis Bacillus Calmette-Guerin infects DC-SIGN- dendritic cell and causes the inhibition of IL-12 and the enhancement of IL-10 production[J]. J Leukoc Biol�� 2005�� 78(1)�� 106-113.[9] FU F�� LI Y�� QIAN S�� et al. Costimulatory molecule-deficient dendritic cell progenitors (MHC class II+�� CD80dim�� CD86-) prolong cardiac allograft survival in nonimmunosuppressed recipients[J]. Transplantation�� 1996�� 62 (5)��659-665.[10] KHAN T A�� PEH K K. A preliminary investigation of chitosan film as dressing for punch biopsy wounds in rats[J]. J Pharm Sci�� 2003�� 6(1)�� 20-26.[11] DELEMARRE F G�� HOOGEVEEN P G�� DE HAAN-MEULMAN M�� et al. Homotypic cluster formation of dendritic cells�� a close correlate of their stage of maturation[J]. J Leukoc Biol�� 2001�� 69 (3)�� 373-380.[12] SAEMANN M D�� PAROLINI O�� BOHMIG G A�� et al. Bacterial metabolite interference with maturation of human monocyte-derived dendritic cells[J]. J Leukoc Biol�� 2002�� 71(2)�� 238 -246.[13] NENCIONI A�� BECK J�� WERTH D�� et al. Histone deacetylase inhibitors affect dendritic cell differentiation and immunogenicity[J]. Clin Cancer Res�� 2007�� 13(13)�� 3933-3941.[14] MILLARD A L�� MERTES P M�� ITTELET D�� et al. Butyrate affects differentiation�� maturation and function of human monocyte-derived dendritic cells and macrophages[J]. Clin Exp Immunol�� 2002�� 130(2)�� 245-255.[15] DIAKOS C�� PRIESCHL E E�� SAEMANN M�� et al. Novel mode of interference with nuclear factor of activated T-cells regulation in T-cells by the bacterial metabolite n-butyrate[J]. J Biol Chem�� 2002�� 277(27)�� 24243-24251.
2011, Vol. 46 
