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�й�ҩѧ��־ 2011, Vol. 46 Issue (24) :1874-1878    DOI:
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LIU Lua�� LI Linc�� MIN Junb�� WANG Jieb�� ZENG Yu-jiea�� WU Henga�� CHEN Shuanga�� CHU Zhong-huaa*
a.Department of Gastrointestinal surgery�� b. Department of Hepato-Biliary Surgery�� c.Department of Obstetrics and Gynecology�� Sun Yat-sen Memorial Hospital of Sun Yat-sen University�� Guangzhou 510120�� China

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ժҪ Ŀ�� ̽�ֲ�ͬŨ�ȶ������յ�����������������ͻ״ϸ����dendritic cells�� DC�����ߴ̼����Ե�Ӱ�졣���� ͨ����������ϸ��-����ϸ������̼�����(GM-CSF)���������ϸ������4��IL-4�������յ����˵�����ϸ����Դ��DC������0.5��0.75��1��1.25��1.5 mmol��L-15��Ũ�ȶ����������յ��ֻ������ֱ�����ʽϸ���ǣ�FITC-dxtran���̼�⡢MLR��ELISA�����DC�ĵ����ʡ������־�������������̼��ܰ�ϸ����ֳ������IL-10��IL-12��INF-�÷��ڵı仯����� 1.25��1.5 mmol��L-1�������нϸߵ�ϸ�����ԡ�0.5��0.75��1 mmol��L-1�����ƾ�������DC���������͵ı�������1.0 mmol��L-1�������յ���DC��������ͱ��Ч�����ȶ�����Ȼ��ͬŨ�ȶ������յ��µ�DC�����������������죨P>0.05������1.0 mmol��L-1��������0.5��0.75 mmol��L-1��������Ƚ���IL-10��������3.29��0.21����������ܰ�ϸ����ֳ������1.53��1.04����ǿ��P<0.05�������� 1.0 mmol��L-1���������յ�DC�ȶ�������״̬�����Ũ�ȡ�
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Abstract�� OBJECTIVE To investigate the immunological effects of different concentrations of sodium butyrate on dendritic cells (DC) differentiation in vitro. METHODS Human monocyte-derived DC were induced in the presence of cytokine recombined human granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4. The effects of different concentrations of sodium butyrate on DC were detected by FCM�� endocytic activity�� T cells stimulatory proliferation capacity�� and IL-10�� IL-12 and INF-�� production. RESULTS Sodium butyrate of 1.25 and 1.5 mmol��L-1 had much higher toxicity on DC compared with sodium butyrate below 1 mmol��L-1. All three concentrations of sodium butyrate below 1 mmol��L-1 could down-regulate the major histocompatibility complex (MHC) class II and costimulatory molecules of DC. Sodium butyrate of 1 mmol��L-1 had better suppression effects on mature markers of DC than other groups (0.5 and 0.75 mmol��L-1)�� although the endocytic activity of DC had no significant differences among the three groups (P>0.05). Sodium butyrate of 1.0 mmol��L-1 had the strongest capacity to induce a stage of T-cell anergy��1.53��1.04��and promote IL-10 production (3.29��0.21) compared with those of 0.5 and 0.75 mmol��L-1 sodium butyrate. CONCLUSION 1.0 mmol��L-1 is the most effective concentration of sodium butyrate for inducing immaturation state of dendritic cells.
Keywords�� dendritic cell,   sodium butyrate,   immunotolerance     
�ո�����: 2011-11-11;
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.��ͬŨ�ȶ������յ�δ������ͻ״ϸ���ֻ�������ѧ���ܱȽ�[J]  �й�ҩѧ��־, 2011,V46(24): 1874-1878
.Comparison of the Immunological Effect of Sodium Butyrate of Different Concentrations on the Differentiation of Dendritic Cells[J]  Chinese Pharmaceutical Journal, 2011,V46(24): 1874-1878
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