a. State Key Laboratory of Bioreactor Engineering�� b. Biology Engineering Department��East China University of Science and Technology��Shanghai 200237��China
Abstract��
OBJECTIVE To construct Escherichia coli producing coenzyme Q10 and investigate the decaprenyl diphosphate synthase expression.METHODS The ddsA gene encoding decaprenyl diphosphate synthase from Rhodobacter capsulatus B10 was cloned downstream different promoters(Lac,T7 and Trc) and heterologous expressed in E.coli JM83.The purification of ddsA fusion protein was investigated with His-tag system. RESULTS E.coli was observed to synthesize coenzyme Q10in vivo.The fusion protein was expressed in supernatant after being cultivating overnight at 28 ��,and was separated by 150 mmol��L-1 imidazole on Ni-sepharose column.CONCLUSION E.coli can produce coenzyme Q10 by heterologous expressing the decaprenyl diphosphate synthase from Rhodobacter capsulatus B10.
.Heterologous Expression and Purification of Decaprenyl Diphosphate Synthase from Rhodobacter capsulatus B10 [J] Chinese Pharmaceutical Journal, 2007,V42(01): 13-16
2007, Vol. 42 