Abstract��
OBJECTIVE To observe the induced apoptosis effect of 5F on HPF cultured in vitro and the expression of caspase-3.METHODS Cell were treated with different concentration of 5F(0,8,32 and 128 mg��L-1)with different time.The effect of 5F on cell proliferation was detected by thiazole blue chromatomentry.Cell apoptosis was observed by Hoechst33258 fluorescent staining;flow cell cycle and apoptosis were investigated by flow cytometry.The transcription level of caspase-3 were determined by RT-PCR;The expression of caspase-3 protein was detected by Western blot;The change of caspase-3��s activity during the process of cell apoptosis was valued by chemistry chromatometry.RESULTS Compared with the control group,the cell growth in experimental group was inhibited significantly(P<0.01),and the cell proliferation inhibition rate showed a dose-and time-dependent manner.Hoechst33258 fluorescent staining displayed that cells in experimental group partly showed typical apoptosis.Under the effect of 5F with the concentration of 32.18 mg��L-1,the peak of apoptosis was reached at 12 h and was(12.48��1.29)%;RT-PCR showed that the expression of caspase-3 mRNA in cells was increased.Western blot showed that the expression of caspase-3 protein was increased.The caspase-3 activity was increased dose-and time-dependerthy.The activity was 3.52 times as control group when the concentration of 5F was 128 mg��L-1.CONCLUSION 5F can inhibit the proliferation of PHF,induce cell apoptosis,increases the activity of caspase-3,and show a dose-and time-dependent manner.The mechanism of 5F inducing cell apoptosis may relate with the increasing of caspase-3 activity,facilitating of transcription of caspase-3 and protein translation,increasing of casepase-3 in order to accelerate the cell apoptosis.
.Experimental Research of Apoptosis in Pterygium Fibroblasts Induced by 5F and Effect on the Expression of Caspase-3 [J] Chinese Pharmaceutical Journal, 2006,V41(13): 993-997
2006, Vol. 41 
