Abstract��
OBJECTIVE To establish a liquid chromatography-electrospray mass spectrometry (LC/ESI-MS) method for the determination and pharmacokinetic study of evodiamine, rutaecarpine in rabbits. METHODS Evodiamine and rutaecarpine in rabbits plasma at different sampling time were determined by HPLC/MS. The plasma samples were deproteined by methanol.The separation was performed on an Agilent Zorbax SB-C18 column with acetonitrile-2% tetrahydorfuran (62:38) as the mobile phase at the flow rate of 0.5 mL��min-1. Electrospray ionization source (ESI) was applied and operated in the negative ion mode. The m/z 302 (evodiamine, [M-H]-), m/z 286 (rutaecarpine , [ M-H]-) and 595 ( rutaecarpine, [ 2M+Na-2H]-) were used to quantify evodiamine and rutaecarpine respectively using the selected ion monitoring (SIM) mode. The pharmacokinetic parameters were calculated with 3P87 pharmacokinetic program.RESULTS The linear range was 0.32��32.0 and 1.0��40.0��g��L-1 for evodiamine and rutaecarpine, respectively. The average extraction recoveries were all over 80% . The inter-and intra-day precisions (RSD) were all less than 5% (n=6). The concentration-time curves of evodiamine and rutaecarpine were fitted to an one-compartment model. The total areas under the plasma concentration-time curve (AUC) were (183.2��76.2) and (453.5��191.0)��g��min��L-1 for evodiamine and rutaecarpine, respectively.The plasma clearances (CLs) of evodiamine and rutaecarpine were (47.2��29.0) and (41.4��23.3) L��kg-1��min-1, respectively.CONCLUSION The method is simple, rapid and sensitive. It is proved to be suitable for pharmacokinetic studies of evodiamine and rutaecarpine.
LUAN Lian-jun,
QIU Guo-li,
CHENG Yi-yu etc
.Study on Pharmacokinetics of Evodiamine and Rutaecarpine in Rabbit [J] Chinese Pharmaceutical Journal, 2006,V41(01): 48-50
2006, Vol. 41 
