1.Department of Biochemistry��Zhongshan Medical College��Sun Yat-Sen University�� Guangzhou 510089��China��2. Qiangji Pharmaceutical Factory�� Chaozhou 515636��China��3. Center of Experimental Animal�� Sun Yat-Sen University��Guangzhou 510089��China��4. Sanpotel Pharmaceutical Co.�� Ltd�� Nanning 530200��China��5.Department of Chemical Carcinogenesis��Guangzhou Medical College�� Guangzhou 510050��China
Abstract��
OBJECTIVE To prepare and study the oral carcinoembryonic antigen (CEA) positive tumor gene vaccine capsules. METHODS By using a cloning method, a EGFP-CEA cDNA fusion gene vaccine pVGC2 was constructed as a tracer in which EGFP cDNA fragment was linked to the upstream of CEA cDNA. With a kind of subminiature hollow capsules which dissolved at the colon site, the oral gene vaccine capsules were prepared for mice. The expression of the protein of the ECFP-CEA gene in mice was estimated after the capsules were administered orally. The green fluorescent features in the free cells collected from the abdominal cavity were observed by fluoromi-croscope. RESULTS The nucleotide sequences of the target gene fragments of the recombinant plasmid were verified that the acquired EGFP cDNA sequence was coincidence with the original cDNA( 100% ) provided. The average dissolution of the oral capsules was (91.1 �� 3.65) % . And the results showed that the free cells had a visible green fluorescence after the oral treatment. CONCLUSION A kind of oral gene vaccine capsules were successfully prepared. And the green fluorescent expression of the fusion protein of the gene vaccine in the cells indicated the oral medicine path of the capsules was a potentially immune way for anticaneer.
2005, Vol. 39 