Abstract��
OBJECTIVE To study protein binding of valproic acid in epileptic patients serum. METHODS Free valproic acid was extracted from the serum of 30 epileptic patients by ultrafiltration. Then the total level and free level of valproic acid in 30 epileptic patients were determined by fluorescence polarization immunoassay, respectively. Finally serum protein binding ratio of valproic acid in epileptic patients was calculated. RESULTS Serum protein binding ratio of valproic acid in 30 patients ranged from 88.78 % to 95.91 % , and the average ratio was 93.63% . Individual variability of serurn protein binding ratio of valproic acid in 30 patients was not obvious( RSD =2.06%).CONCLUSION A high serum protein binding of valproic acid was found in patients.The level of valproic acid will be comprehensively considered in clime. It is suggested that the blood drug level of free valproic acid should be monitored in suitable occassion.
2005, Vol. 39 
