目的　利用正交实验研究吴茱萸中吴茱萸碱和吴茱萸次碱的最佳提取条件。方法　首先研究提取溶剂和提取方法对吴茱萸碱和吴茱萸次碱提取效率的影响。然后设计正交实验 ,安排提取时间、提取温度、溶媒倍数3个因素,采用L₉(3⁴)正交实验表 ,HPLC法检测,色谱柱 :YWG-C₁₈(10μm,4.6mm×250mm)。流动相:水 -甲醇-乙腈(42∶20∶38)。流速:1.0mL·min^-1。柱温:30℃。检测波长:260nm。结果　醋酸乙酯提取效率较高,水、酸水和碱水不能提出吴茱萸碱和吴茱萸次碱;超声震荡法提取效率较高 ;吴茱萸碱和吴茱萸次碱的最佳提取条件为用45倍溶媒于30℃下超声提取40min。结论　用本实验方法提取 ,效率高 ,时间短 ,所需温度低 ,是提取吴茱萸碱和吴茱萸次碱较好的方法。

OBJECTIVE The optimum extraction conditions of evodiamine and rutaecarpine from herbs Evodia rutaecarpa(Juss.)Benth were studied by means of orthogonal test.METHODS The contents of evodiamine and rutaecarpine were determined by high performance liquid chromatography (HPLC). The extraction efficiency of extraction methods were compared. The solvent ethyl acetate and the ultrasonic method were better Then the orthogonal test L₉(3⁴) was arranged with three factors which were extraction time, extraction temperature and solvent times. The separation was performed on YWG-C₁₈(4.6mm×250mm,10μm) column with water-methanol acetonitrile (42∶20∶38)as mobile phase.The flow rate was 1.0 mL·min^-1,the column temperature was 30℃ and the wavelength of UV detector was 260 nm. RESULTS The solvent ethyl acetate and the method of ultrasonic were better.Water, acid and alkali were not appropriate to extract evodiamine and rutaecarpine. The optimum extraction conditions were decided to be at 30℃ for 40 min with 45 times solvent by ultrasonic. CONCLUSION The method had higher extraction efficiency as well as shorter time and lower temperature, it was a better method for extracting evodiamine and rutaecarpine from Evodia rutaecarpa (Juss.)Benth.