目的 探讨丹参的脂溶性有效成分(总丹参酮)对血管平滑肌细胞增殖的影响及其可能的细胞内信号传导机制。方法 分离大鼠胸主动脉,贴壁法培养平滑肌细胞,无血清培养基培养静止后,以5%FBS刺激VSMC增殖,分别加入不同浓度的总丹参酮,以MTT及细胞总蛋白测定法观察药物对VSMC增殖的影响;用Westernblot方法检测pp-Erk1/2蛋白表达量。结果 丹参酮对VSMC增殖具有抑制作用,并具有浓度依赖性;2,10,50μg·mL-1丹参酮可浓度依赖性抑制pp-Erk1/2表达,抑制率分别为(17.7±3.6)%,(26.2±4.1)%,(72. 7±3.8)%,与对照组相比P<0.05。结论 丹参的脂溶性有效成分丹参酮可能通过抑制ERK途径抑制血管平滑肌细胞增殖。
Abstract
OBJECTIVE To investigate the effects of tanshinone on rat vascular smooth muscle cell(VSMC) proliferation and ERK signaling.METHODS Rat dorsal aortic VSMCs were cultured by tissue sticking method.MTT and protein assay were used to estimate the proliferation activity of VSMC. Western blot was used to assess the protein expression of pp-ERK1/2. RESULTS Tanshinone inhibited VSMC proliferation response stimulated by 5% fetal bovine serum (FBS) and serum stimulated pp-ERK1/2 expression in a dose dependent fashion. Inhibition rates of 2,10,50μg·mL-1 tanshinone were (17.7±3.6)%,(26.2±4.1)% and (72.7±3.8)%(P <0.05 vs control), respectively. CONCLUSION The results suggested that tanshinone down regulated the expression of pp-ERK1/2, which might be the mechanism in depressing VSMC proliferation.
关键词
丹参酮 /
血管平滑肌细胞 /
增殖 /
细胞外信号调节蛋白激酶
{{custom_keyword}} /
Key words
tanshinone /
vascular smooth muscle cell(VSMC) /
proliferation /
extracellular signal regulated kinase(ERK)
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]张文军,包晓峰,王秀凤，等.丹参酮Ⅱa磺酸钠抑制巨噬细胞源性生长因子刺激平滑肌细胞c-myc表达[J\].中国动脉硬化杂志,1996,4(1)：45.
[2]Weissberg PL, Bennett MR, Rose R. Atherosclereosis-an inflammatory disease[J\].N Eng J Med,1999,340(2)：115.
[3]Mii S, Khalil RA, Morgan KG, et al. Mitogen-activated protein kinase and proliferation of human vascular smooth muscle cells[J\].Am J Physiol,1996，270： H142.
[4]Pelech SL, Sanghera JS.MAP kinases： charting the regulatory pathways[J\].Science,1992，257：1355.
[5]Koyama H, Olson NE, Dastvan FF, et al. Cell replication in the arterial wall： activation of signaling pathway following in vivo injury[J\].Circ Res,1998，82：713.
[6]黄熙，藏益民．丹参酮Ⅱa磺酸钠心血管药理[J\].国外医学·中医中药分册，1995, 17(1)：9.
[7]徐长庆，郝雪梅．丹参酮ⅡA对豚鼠单个心室肌细胞跨膜电位及L-型钙电流的影响[J\].中国病理生理杂志,1997,13(1)：43.
[8]陶军，王舟琪，刘桥义,等．丹参酮防治心肌再灌注损伤的实验研究[J\].中华麻醉学杂志,1996,16(5)：202.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家重点基础研究“973”发展规划项目(1999054403)
{{custom_fund}}
