Abstract��
OBJECTIVE: To investigate the effects of dl-3-n-butylphthalide (dl-NBP),d-NBP and l-NBP on the activity of choline acetyltransferase (ChAT) in ischemic brain after focal cerebral ischemia and in neurons subjected to hypoglgcemia/hypoxia. METHODS: Focal cerebral ischemia was induced by blockade of the origin of middle cerebral artery (MCAO) and perinatal cortical rat neurons was cultured with hypohlycemia/hypoxic medium to induce hypoglycemia/hypoxia injury.The activity of ChAT was determined spectrophotometrically. RESULTS: The results indicated that the activity of ChAT was decreased by 61.3% and 58.4% respectively in cerebral cortex and striatum after six hours of MCAO.dl-NBP,d-NBP and l-NBP (10 and 20mg��kg-1,ip) were shown to increase ChAT activity in ischemic brain significantly.The decreased activity of ChAT in cultured perinatal rat cortical neurons induced by hypoglycemia/hypoxia or by NMDA was also improved by dlNBP,dNBP and lNBP (0.1��10mol��L-1). CONCLUSION: The effect of dl-NBP on learning and memory function impaired by focal cerebral ischemia may be related to its protective effect on the activity of choline acetyltransferase.
Chong Zhaozhong,
Chong Zhaozhong
.Effect of dl-3-n-butylphthalide on the activity of the choline acetyltransferase in ischemic brain and cultured neurons subjected to hypoglycemia/hypoxia[J] Chinese Pharmaceutical Journal, 1999,V34(08): 519-522
1999, Vol. 34 
