Abstract��
OBJECTIVE: To investigate the diterpenoid constituents and anticancer action of the pteris semipinnata L.(PSL). METHOD:TBZThe ethanol extract was fractionated over an active charcoal column,eluted with ethanol-chloroform(10:1).After concentrating,the remainder was chromatographed on sillica gel column,eluted with chloroform-methanol (90:2).The pure compounds were obtained.Their structures were confirmed by spectra,espectially by two-dimensional nuclear magnetic resonance (2D-NMR) for their stereo structures.Cell viability was determined using the 3-(4,5-Dimethyl-2-thia-zolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) test,and the IC50 value were calculated by the modified Karber formula. RESULTS: Five compounds,B,4F,5F,6F and A ,were obtained.A is a new compuound,6F was isolated from the plant for the first time.A and 6F exhibited obvious activity to inhibit the growth of human promyelocytic leukemia cells (HL-60),gastric adenocarcinom cell line (MGC-803),human nasopharyngeal carcinoma cells in low differentiation (CNE-2Z) and human liver adenocarcinoma cell line (BEL-7402),6F is more active than the positive control by 5Fu,their IC50 for LC-60 were 0.09��mol��L-1 and 52.5��mol��L-1,respectively. CONCLUSION: The diterpenoids obtained from the PSL possess obvious antitumour activity.The preliminary study on the structural-activity relationship suggested that the cytotoxic activity is related to the a-methylene cyclopentanone moity.The 6F is the most active one.
Zhang Xiao,
Zhang Xiao
.Study on the diterpenoid constituents and anticancer action of pteris semipinnata[J] Chinese Pharmaceutical Journal, 1999,V34(08): 512-514
1999, Vol. 34 
