WANG Jian-hua1,2��ZHANG Min1��GAN Lu1��ZHANG Sheng-hua1��
1.Department of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070;2.College of Sciene,Shandong Agricultural University,Tai��an 271018,China
Abstract��
OBJECTIVE To study the scavenging effect of LBP1 (one component of polysaccharides from Fructus Lycii,the fruit of ��Lycium barbarum L.) on hydroxyl radicals (��OH),and the protective effect of LBP1 on the mice liver mitochondria injured by ��OH.METHODS ��OH were generated from Fe2++H2O2 or Fe2++VC.The scavenging effect of LBP1 on ��OH was detected by salicylic acid method.To study the protective effect of LBP1,the content of MDA was measured by TBA method.The swelling extents of mice liver mitochondria were detected by spectrophotometric method,and the membrane fluidity of mice liver mitochondria was measured by the fluorescence polarization assay with DPH fluorescence probe.RESULTS LBP1 had the ability to scavenge��OH,decrease the generation of MDA,inhibit the decrease of mitochondria fluidity induced by ��OH,and alleviate the swelling of mitochondria induced by ��OH.CONCLUSION LBP1 had the effects of scavenging ��OH and inhibiting the mice liver mitochondria injury induced by ��OH.
.Protective effect of polysaccharide-1 from Fructus lycii on mice liver mitochondria injury induced by hydroxyl radicals[J] Chinese Pharmaceutical Journal, 2001,V36(10): 669-672
2001, Vol. 36 
