枸杞多糖-1对羟自由基所致小鼠肝线粒体损伤的作用

王建华;张民;甘璐;张声华;

中国药学杂志 ›› 2001, Vol. 36 ›› Issue (10) : 669-672.

中国药学杂志 ›› 2001, Vol. 36 ›› Issue (10) : 669-672.
药理

枸杞多糖-1对羟自由基所致小鼠肝线粒体损伤的作用

  • 王建华,;张民;甘璐;张声华;
作者信息 +

Protective effect of polysaccharide-1 from Fructus lycii on mice liver mitochondria injury induced by hydroxyl radicals

  • WANG Jian-hua1,2;ZHANG Min1;GAN Lu1;ZHANG Sheng-hua1;
Author information +
文章历史 +

摘要

目的 研究枸杞多糖(lycium barbarum polysaccharides,LBP)组分1(LBP1)清除羟基自由基(·OH)的效果及其对·OH所致小鼠肝线粒体氧化损伤的作用效果。方法 用Fe2++H2O2或Fe2++VC系统产生·OH;用水杨酸法研究LBP1清除·OH的效果;用硫代巴比妥酸法测定小鼠肝线粒体丙二醛含量、用以DPH为荧光探剂的荧光偏振法测定小鼠肝线粒体膜流动性、以及用分光光度法测定小鼠肝线粒体膨胀程度研究LBP1抗·OH氧化损伤的效果。结果 LBP1可以清除·OH,减少·OH所致丙二醛的产生,抑制·OH所致膜流动性下降和减轻·OH所致肝线粒体膨胀程度,并呈量效关系。结论 LBP1具有清除·OH和抑制·OH所致小鼠肝线粒体氧化损伤的作用。

Abstract

OBJECTIVE To study the scavenging effect of LBP1 (one component of polysaccharides from Fructus Lycii,the fruit of Lycium barbarum L.) on hydroxyl radicals (·OH),and the protective effect of LBP1 on the mice liver mitochondria injured by ·OH.METHODS ·OH were generated from Fe2++H2O2 or Fe2++VC.The scavenging effect of LBP1 on ·OH was detected by salicylic acid method.To study the protective effect of LBP1,the content of MDA was measured by TBA method.The swelling extents of mice liver mitochondria were detected by spectrophotometric method,and the membrane fluidity of mice liver mitochondria was measured by the fluorescence polarization assay with DPH fluorescence probe.RESULTS LBP1 had the ability to scavenge·OH,decrease the generation of MDA,inhibit the decrease of mitochondria fluidity induced by ·OH,and alleviate the swelling of mitochondria induced by ·OH.CONCLUSION LBP1 had the effects of scavenging ·OH and inhibiting the mice liver mitochondria injury induced by ·OH.

关键词

枸杞 / 多糖 / 线粒体 / 羟基自由基 / 氧化损伤

Key words

Lycium barbarum / polysaccharide / mitochondria / hydroxyl radical / oxidative damage / anti-oxidation

引用本文

导出引用
王建华;张民;甘璐;张声华;. 枸杞多糖-1对羟自由基所致小鼠肝线粒体损伤的作用[J]. 中国药学杂志, 2001, 36(10): 669-672
WNG Jin-hu;ZHNG Min;GN Lu;ZHNG Sheng-hu;. Protective effect of polysaccharide-1 from Fructus lycii on mice liver mitochondria injury induced by hydroxyl radicals[J]. Chinese Pharmaceutical Journal, 2001, 36(10): 669-672

参考文献

[1]田庚元.中药免疫调节剂的研究和开发[J].科学中国人,1999,10:43. [2]白寿宁.宁夏枸杞研究[M].银川:宁夏人民出版社,2000:681~1100. [3]何进,张声华.枸杞多糖的分离纯化及组成研究[J].中国药学杂志,1996,31(12):716. [4]黄琳娟,林颖,田庚元,等.枸杞子中免疫活性成分的分离.纯化及物理化学性质的研究[J].药学学报,1998,33(7):512. [5]罗琼,阎俊,李瑾玮,等.枸杞多糖粗品与纯品抗疲劳作用比较[J].营养学报,1999,21(3):310. [6]张熙,谢先春.枸杞多糖抗氧化作用的实验研究[J].中国中药杂志,1993,18(2):110. [7]赵春久,李荣芷,何云庆,等.枸杞多糖的化学研究[J].北京医科大学学报,1997,29(3):231. [8]赵保路.氧自由基和天然抗氧化剂[M].北京:科学出版社,1999:77~153. [9]孙智达.功能因子LBP的研究—LBP的结构及理化性质[D].武汉:华中农业大学博士论文,1997:25~26. [10]李志孝,黄成钢,蔡育军,等.天门冬多糖的化学结构及体外抗氧化活性[J].药学学报,2000,35(5):358. [11]Smirnoff N,Cumbes QJ.Hydroxyl radical scavenging activity of compatible solutes[J]. Phytochemistry, 1989,28(4):1057. [12]向荣,王鼎.过氧化脂质硫代巴比妥酸分光光度法的改进[J].生物化学与生物物理进展,1990,17(3):241. [13]Bowling AC,Mutisya EM,Walker LC.Agedependent impairment of mitochondria function in primate brain[J]. J Neurochem, 1993,60(5):1964. [14]路雪雅.抗坏血酸和硫酸亚铁诱导鼠肝线粒体损伤的实验研究[J].生物化学与生物物理进展,1989,16(5):372.

Accesses

Citation

Detail

段落导航
相关文章

/