Abstract��
OBJECTIVE To establish a RP-HPLC method for simultaneous determination of six kinds of triterpenoid acids in Ganoderma,i.e.ganoderic acid A,ganoderic acid B,ganoderic acid C2,ganoderic acid G,ganoderic acid E and lucidenic acid A.METHODS The separation was performed on a Diamonsil-C18 column(4.6 mm��250 mm,5 ��m),using a gradient elution with acetonitrile-wate(rcontaining 0.8% acetic acid)as the mobile phase.The flow rate was 1 mL��min-1,the detection wavelength was at 254 nm and the temperature of column was at room temperature.RESULTS The linear relationship were obtained within the range of 0.650-7.800,0.175-2.100,0.206-2.475,0.188-2.250,0.275-3.300,0.120-1.440 ��g for the six components respectively.The average recoveries of the six components(n=6) were 100.06%(RSD=2.6%),99.55 %(RSD=1.6 %),100.75%(RSD=1.5 %),98.86 %(RSD=1.9 %),99.26%(RSD=2.3%) and 98.95%(RSD=1.3%).CONCLUSION The simple,rapid and reliable method could be used for the quality control of Ganoderma.
2009, Vol. 44 