1.Department of Laboratory Medicine��Huaihua Medical College��Huaihua 418000��China��2.Center for Structural and Molecular Biology��Institute of Biophysics��Chinese Academy of Sciences��Beijing 100101��China
Abstract��
OBJECTIVE To investigate the activation of nuclear factor-kB(NF-KB)and the expression of inflammatory cytokines in lipopolysaccharide-induced murine peritoneal macrophages stimulated with 2-S[2-(N-carbonyl-3-��-aminoethyl-5-hydroxyindole)-1- (��-tocopheryl-6-yloxy-carbonyl)ethyl]glutathione(EseroS-GS).METHODS Peritoneal macrophages cultured in vitro were given with LPS or EseroS-GS+LPS.The NF-kB activity of peritoneal macrophages was tested by Western blotting and electrophoretic mobility shift assay(EMSA).The expression of TNF-��,IL-1��,IL-6 in peritoneal macrophages were measured by reverse transcription polymerase chain reaction techniques(RT-PCR)and enzyme-linked immuno sorbent assay(ELISA).RESULTS The activity of NF- kB and the level of TNF-��,IL-1��,IL-6 was significantly increased after LPS stimulation for 2 to 12 h(P<0.01).Compared with LPS- stimulated group,both NF-kB activity and concentration of TNF-��,IL-1��,IL-6 were significantly lowered in EseroS-GS+LPS group (P<0.01).CONCLUSION LPS Might activate NF-kB in peritoneal macrophages and induce the increase of transcription and expression of TNF-��,IL-1��,IL-6 genes.EseroS-GS could inhibit the activation of NF-kB and regulate the expression of TNF-��,IL-1��and IL-6 genes in peritoneal macrophages.
ZHANG Shen,
YIN Li-hua,
WEI Tao-tao etc
.Effects of EseroS-GS on Production of Pro-Inflammatory Factors from Lipopolysaccharide Activated Murine Peritoneal Macrophages [J] Chinese Pharmaceutical Journal, 2008,V43(14): 1070-1073
2008, Vol. 43 