Abstract��
OBJECTIVE To prepare the binary complex of cationic solid lipid nanoparticles/pDNA(SLNs-pDNA)and investigate the pharmaceutical and biological characteristics of the complex.METHODS Cationic solid lipid nanoparticles(SLNs) was prepared by double emulsion method,and the reported gene was condensed using the cationic SLNs via electrostatic force leading to the formation of the SLNs-pDNA complex.The electric quantity of the system was modified by addition of various concentrations of Ca2+.The physicochemical properties,in vitro cytotoxicity,release behaviour as well as protection of the SLNs-pDNA complex to pDNA from nuclease degradation were evaluated respectively.Transfection activity of SLN-pDNA complex was determined by gene transfection experiment in vitro in COS-7 cells.RESULTS The morphology of SLNs and the SLNs-pDNA complex were approximately spherical,the average particle sizes of SLNs and the complex were(51.9��0.58)and(91.6��5.3)nm,respectively.The Zeta potentials were(47.4��1.1)and(31.5��1.4)mV,respectively.The cell viability assay showed nanoparticles exhibited a low cytotoxicity to COS-7 cells.The nuclease degradation test results confirmed that the pDNA was protected considerably.SLNs-pDNA maintained sustained-release of pDNA for several days in vitro.CONCLUSION SLNs-pDNA complex could be prepared easily with small particle sizes,low cytotoxicity and high protection to pDNA.The gene transfection experiment in vitro suggested that SLN-pDNA complex could transfer the loaded gene into COS-7 cells,and the gene could express well inside the cells.The complex could be a promising non-virus nano-device,which has the potential to make in vivo cancer gene therapy achievable.
LIU Chun-xi,
YE Jie-sheng,
ZHANG Na etc
.Preliminary Studies on Cationic Solid Lipid Nanoparticles/pDNA Binary Complex [J] Chinese Pharmaceutical Journal, 2008,V43(14): 1050-1055
2008, Vol. 43 