Quality Difference Characteristics of Bupleurum Formulae Granules Based on the Analysis of Dual-Wavelength Fingerprint Profiles and Chemometric Methods
WANG Xiao-wei1, WANG Yan-wei1, WANG Hai-bo1, LIU Rui-xin2, SHI Yan3*
1. Henan Provincial Institute of Food and Drug Control, Zhengzhou 450008, China; 2. The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000, China; 3. National Institutes for Food and Drug Control, Beijing 100050, China
Abstract：OBJECTIVE To distinguishing the Bupleurum formulae granules from different origins and promoting the consistency of quality of them,with the analysis of fingerprint profiles and chemometric methods. METHODS In the study, a method of dual-wavelength (210 & 254 nm) HPLC fingerprint of Bupleurum formulae granules was developed. Based on the fingerprint method, the quality differences of the samples of Bupleurum formulae granules were analyzed by similarity analysis, hierarchical cluster analysis, principal component analysis and partial least squares discriminant analysis. RESULTS The fingerprints of 12 batchs of Bupleurum formulae granules are established. The results indicated that the fingerprints of Bupleurum formulae granules from different origins could be differentiated well and the characteristic peaks could be marked. CONCLUSION The 210 nm fingerprints of Bupleurum formulae granule have the most characteristic differences; It is recommended that the dual-wavelength fingerprints could be combined for analysis and application. Saikosaponin a, saikosaponin b2 and saikosaponin b1 are the key components of the quality difference, which should be focused on in the quality evaluation and control of Bupleurum formula granules.
王晓伟, 王艳伟, 王海波, 刘瑞新, 石岩. 指纹图谱轮廓分析结合化学计量对柴胡配方颗粒质量差异特征性分析[J]. 中国药学杂志, 2020, 55(3): 218-227.
WANG Xiao-wei, WANG Yan-wei, WANG Hai-bo, LIU Rui-xin, SHI Yan. Quality Difference Characteristics of Bupleurum Formulae Granules Based on the Analysis of Dual-Wavelength Fingerprint Profiles and Chemometric Methods. Chinese Pharmaceutical Journal, 2020, 55(3): 218-227.
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2020, Vol. 55 
