Chemical Constituents from the Roots of Scrophularia ningpoensis Hemsl and Their Anti-inflammatory Activities
HUO Yu-feng1, ZHAN Zhi-lai2, WANG Huai-li1*, WEI Er-hu1, JIA Pei-sheng1, LIU Hui-qiong1, FAN Xiao-lei1, YAN Kun-li1
1. Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;
2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
OBJECTIVE To study the chemical constituents in the roots of Scrophularia ningpoensis Hemsl. METHODSE Compounds were isolated by chromatographic techniques and their structures were elucidated by spectral methods. The anti-inflammatory activities of all isolated compounds were evaluated by lipopolysaccharides (LPS)-stimulated BV2 cells model. RESULTS Eighteen compounds were isolated and identified as 2'''-acetyl angroside C (1), saccatoside (2), 6-O-α-L-(2″-O-feruloyl)rhamnopyranosylcatalpol (3), scrophularioside (4), harprocumbide A (5), 6″-O-β-D-glucopyranosylharpagoside (6), harpagoside (7), 8-O-(p-coumaroyl)-harpagide (8), 8-O-feruloylharpagide (9), 6-O-α-D-galactopyranosylharpagoside (10), 6′-O-cinnamoylharpagide (11), angoroside B (12), angoroside C (13), scrophuloside B1 (14), 2-(3-hydroxy-4-methoxyphenyl)ethyl-O-α-L-arabinopyranosyl-(1→6)-O-α-L-rhamnopyranosyl-(1→3)]-β-D-glucopyranoside (15), darendoside B (16), 6-O-caffeoyl-β-D-fructofuranosyl-(2→1)-α-D-glucopyranoside (17), and sibirioside A (18). CONCLUSION Compound 1 is new compound, and compounds 2-4, 12, 14, and 17 are isolated from this plant for the first time. Compounds 1, 12, and 13 show significant inhibition against nitric oxide production in LPS-stimulated BV2 cells model.
霍玉峰, 詹志来, 王怀立, 魏二虎, 贾沛生, 刘会琼, 范晓蕾, 闫坤丽. 中药玄参中化学成分及其抗炎活性研究[J]. 中国药学杂志, 2020, 55(14): 1207-1214.
HUO Yu-feng, ZHAN Zhi-lai, WANG Huai-li, WEI Er-hu, JIA Pei-sheng, LIU Hui-qiong, FAN Xiao-lei, YAN Kun-li. Chemical Constituents from the Roots of Scrophularia ningpoensis Hemsl and Their Anti-inflammatory Activities. Chinese Pharmaceutical Journal, 2020, 55(14): 1207-1214.
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2020, Vol. 55 
