Effects of Effective Part of Tinospora sinensis on Hippocampus Proteomics of AD Rats Induced by Beta-amyloid Protein and D-galactose
SUN Meng-sheng1,2, WEI Yi-fei3, HE Kun1, CHEN Yan1, BAI Li-hua1, ZHU Hong-ling1, YANG Qing1, HUANG Hui-lian2, HUANG Li-ping1*
1. Jiangxi University of Traditonal Chinese Medicine,Nanchang 330004, China; 2. Key Laboratory of Modern Preparation of TCM, Ministry of Education, Nanchang 330004, China; 3. Department of Pharmacy, Zhengjiang Hospital, Hangzhou 310013, China
Abstract：OBJECTIVE To investigate the effect of the 80% ethanol elution part of Tinospora sinensis macroporous resin extract on the expression of hippocampus proteome in Alzheimer′s disease (AD) model rats induced by D-galactose combined with Aβ25～35. METHODS The AD rats model replicated by D-galactose combined with Aβ25～35, The AD rat model was replicated by D-galactose combined with Aβ25～35, and randomly divided into sham operation group, model group, Donepezil group (donepezil, 6.0 mg·kg-1), and 80% extraction of Tinospora sinensi group (crude drug 6 g·kg-1). Donepezil group: donepezil 0.1 mL·10 g-1 ig. 80% extraction of Tinospora sinensi group: Tinospora sinensis effective part extraction 0.1 mL·10 g-1 ig. Model group and sham-operation group: physiological saline 0.1 mL·10 g-1 ig. Once a day, continuous administration for 15 d. Separating the hippocampus and extracting the protein, take the system test with nanol-ESI liquid-mass spectrometry, protein discovery software was used for identification, and qualitative analysis different groups of hippocampal proteins by SIEVE software. Take the GO analysis on differential protein with the ANTHER classification system and use IPAD to enrich the pathway. RESULTS Compared with the model group, the drug-administered group had 66 differential proteins, including tubulin, heat shock proteins, energy metabolism-related proteins, vesicle production/transport related proteins, and brain protection-related proteins, which are closely related to AD. The above differential proteins involve a total of 21 signaling pathways. CONCLUSIONTinospora sinensis may promote the synthesis and release of neurotransmitters by up-regulating clathrin and vesicle-forming transport and neurotransmitter release, and improve the function of cholinergic function in the brain to achieve the pathological process of AD.
孙梦盛, 韦益飞, 何堃, 陈燕, 白立华, 朱泓凌, 杨青, 黄慧莲, 黄丽萍. 宽筋藤有效部位对D-半乳糖联合Aβ25～35所致AD大鼠海马蛋白组学的影响[J]. 中国药学杂志, 2020, 55(1): 19-25.
SUN Meng-sheng, WEI Yi-fei, HE Kun, CHEN Yan, BAI Li-hua, ZHU Hong-ling, YANG Qing, HUANG Hui-lian, HUANG Li-ping. Effects of Effective Part of Tinospora sinensis on Hippocampus Proteomics of AD Rats Induced by Beta-amyloid Protein and D-galactose. Chinese Pharmaceutical Journal, 2020, 55(1): 19-25.
BAI W T, XIE Y H, WEI Y F, et al. Chemical constituents of tibetan Tinospora sinensis polar parts. Chin Med Mater(中药材), 2017, 40(3):600-603.
[2]
HOU M, FU Y J, LIU C, et al. Effects of extraction from raspberry on hippocampus proteomics of mice suffered from ovariectomized-induced AD. China J Chin Mater Med(中国中药杂志), 2016, 41(15):2895-2900.
[3]
KUAI X X, YU J C, ZHANG L. Advance in drug development for Alzheimer′s disease targeted microtuble associated protein Tau. Chin J Rehabil Theory Pract(中国康复理论与实践),2014,20(3):240-244.
[4]
QURESHI H Y, LI T, MACDONALD R, et al. Interaction of 14-3-3ζ with microtubule-associated protein Tau within Alzheimer′s disease neurofibrillary tangles. Biochemistry, 2013, 52(37):6445-6455.
[5]
WU Y M, ZHAO J, MA T, et al. Effects of Tianqi Yizhi granules on mitochondria of brain tissues and energy metabolism in Alzheimer rats. Chin J Inf Tradit Chin Med(中国中医药信息杂志), 2015, 22(5):60-64.
[6]
STEEN E, TERRY B M, RIVERA E J, et al. Impaired insulin and insulin-like growth factor expression and signaling mechanisms in Alzheimer′s disease-is this type 3diabetes?. J Alzheimer′s Dis, 2005, 7(1):63-80.
[7]
TAN Z Q, LI F, LIU R Z, et al. Study on heat shock protein in Alzheimer′s disease. Chin J Cell Biol(细胞生物学杂志), 2005,27(4):369-373.
[8]
HOU H, WANG J Z, LI X M, et al. The role of the peptidyl-prolyl cis/trans isomerase Pin1 in the occurrence and development of Alzheimer′s disease. Prog Biochem Biophys(生物化学与生物物理进展), 2015,42(5):434-442.
[9]
LI M F, SHI Y. The mechanism of clathrin-mediated synaptic vesicle endocytosis. Neurosci Bull(中国神经科学杂志), 2002, 18(3):643-647.
[10]
HACKMANN Y, GRAHAM S C, EHL S, et al. Syntaxin binding mechanism and disease-causing mutations in Munc18-2. Proc Natl Acad Sci USA, 2013, 110(47):4482.
2020, Vol. 55 
