Study on Substitute Reference Substance Method with DRS Origin Software: A Case Study of Determination of 11-keto-β-boswellic Acid and 11-keto-β-acetyl-boswellic Acid in Frankincense by UHPLC
SUN Cai-lin, SUN Lei*, WANG Zhao, JIN Hong-yu, MA Shuang-cheng*
National Institutes for Food and Drug Control, Beijing 100050, China
Abstract：OBJECTIVE To establish a UHPLC method for determination of the contents of 11-keto-β-boswellic acid(KAB) and 11-keto-β-acetyl-boswellic acid(AKBA)in Frankincense and explore the suitability and accuracy of substitute reference substance method with DRS origin software for qualitative and quantitative determination of chromatographic peaks. METHODS The samples were separated by UHPLC for determination of AKBA and KBA. AKBA was used as a reference to investigate the accuracy of KBA identification using DRS origin software on 19 different C18 columns. The RSDs of relative correction factors were calculated for different detection wavelengths and instruments.The relative correction factor method and the external standard method were selected for quantification and the differences were compared. RESULTS The established method met the requirements of methodology and the average recovery was 100.21%(n=6) with RSD of 2.47%. The DRS origin software can be used to accurately determine the chromatographic peaks. The correct factor of AKBA vs. KBA was 0.936 and it was consistent under different conditions. There were no significant differences between the content calculated by the relative correction factor method and by the external standard method. CONCLUSION This method is intelligent, feasible, reliable and economical, and can be used for the determination of frankincense content.
孙彩林, 孙磊, 王赵, 金红宇, 马双成. 基于DRS origin的替代标准物质法研究——以超高效液相色谱测定乳香中11-羰基-β-乳香酸和11-羰基-β-乙酰乳香酸的含量为例[J]. 中国药学杂志, 2019, 54(17): 1411-1417.
SUN Cai-lin, SUN Lei, WANG Zhao, JIN Hong-yu, MA Shuang-cheng. Study on Substitute Reference Substance Method with DRS Origin Software: A Case Study of Determination of 11-keto-β-boswellic Acid and 11-keto-β-acetyl-boswellic Acid in Frankincense by UHPLC. Chinese Pharmaceutical Journal, 2019, 54(17): 1411-1417.
SUN L,XU J M,JIN H Y,et al. Herbalism, botany and components analysis study on original plants of Frankincense[J]. China J Chin Mater Med(中国中药杂志),2011,36(2):112-116.
[2]
Ch.P(2015)Vol Ⅰ(中国药典2015年版. 一部)[S]. 2015:1-223.
[3]
AL-HARRASI A,HUSSAIN H,HUSSAIN J,et al. Two pyrolysate products from Omani Frankincense smoke: first evidence of thermal aromatization of boswellic acids[J]. J Anal App Pyrolysis,2014,110(1):430-434.
[4]
VUDDANDA P R,SINGH S,VELAGA S. Boswellic acid-medicinal use of an ancient herbal remedy[J]. J Herb Med,2016,6(4):163-170.
[5]
BEGHELLI D, ISANI G, RONCADA P, et al. Antioxidant and ex vivo immune system regulatory properties of Boswellia serrata extracts[J]. Oxid Med Cell Longev,2017,2017(3):1-10.
[6]
MORIKAWA T,MATSUDA H,YOSHIKAWA M. A review of anti-inflammatory terpenoids from the incense Gum Resins Frankincense and Myrrh[J]. J Oleo Sci,2017,66(8):805-814.
[7]
MANNINO G, OCCHIPINTI A, E. MAFFEI M. Quantitative determination of 3-O-acetyl-11-keto-β-boswellic acid (AKBA) and other boswellic acids in Boswellia sacra Flueck (s-yn. B. carteri Birdw) and Boswellia serrata Roxb[J]. Molecules,2016,21(10):1329-1336.
[8]
SHARMA N, BHARDWAJ V, SINGH S,et al. Simultaneous quantification of triterpenoic acids by high performance liquid chromatography method in the extracts of gum resin of Boswellia serrataobtained by different extraction techniques[J]. Chem Cent J, 2016,10(1):49-58.
[9]
AL-HARRASI A, REHMAN N U, MABOOD F,et al. Application of NIRS coupled with PLS regression as a rapid, non-destructive alternative method for quantification of KBA in Boswellia sacra[J]. Spectrochim Acta Part A Mol Biomol Spectrosc, 2017,184(5):277-285.
[10]
BERTHOLD, BÜCHELE, THOMAS S. Analysis of 12 different pentacyclic triterpenic acids from Frankincense in human plasma by high-performance liquid chromatography and photo-diode array detection[J]. J Chromatogr B,2003,795(2):355-362.
[11]
BÜCHELE B, ZUGMAIER W, SIMMET T. Analysis of pentacyclic triterpenic acids from frankincense gum resins and related phytopharmaceuticals by high-performance liquid chromatography. Identification of lupeolic acid,a novel pentacyclic triterpene[J]. J Chromatogr B,2003,791(1):21-30.
[12]
ZHANG C, SUN L, TIAN R T, et al. Combination of quantitative analysis and chemometric analysis for the quality evaluation of three different Frankincenses by ultra high performance liquid chromatography and quadrupole time of flight mass spectrometry[J]. J Sep Sci,2015,38(19):3324-3330.
[13]
SUN L, ZHANG C, TIAN R T, et al.A holistic strategy of identification and quality evaluation of Frankincense by chromatographic fingerprint combined with chemometrics[J]. Chin Pharm J(中国药学杂志),2015,50(2):140-146.
[14]
GAO X Y,JIANG Y,LU J Q, et al. One single standard substance for the deter-mination of multiple anthraquinone derivatives in rhubarb using high-performance liquid ch-romatography-diode array detection[J]. J Chromatogr A,2009,1216(11):2118-2123.
[15]
WANG C Q, JIA X H, ZHU S,et al. A systematic study on the in uencing parameters and improvement of quantitative analysis of multi-component with single marker method using notoginseng as research subject[J]. Talanta,2015,134(3):587-595.
[16]
XIE J, LI J, LIANG J,et al. Determination of contents of catechins in oolong te-as by quantitative analysis of multi-components via a single marker (QAMS) method[J]. Food Analytical Methods,2017,10(2):363-368.
[17]
CUI L, ZHANG Y, SHAO W,et al. Analysis of the HPLC fingerprint and QAMS from Pyrrosia species[J]. Ind Crops Prod,2016,85(7):29-37.
[18]
LI D W, ZHU M, SHAO Y D,et al. Determination and quality evaluation of green tea extracts through qualitative and quantitative analysis of multi-components by single marker(QAMS)[J]. Food Chem, 2016,197(4):1112-1120.
[19]
SUN L, JIN H Y, MA S C,et al. Guideline of substitute reference substance method for evaluation of traditional Chinese medicines[J]. Chin Pharm J(中国药学杂志),2015,50(4):284-286.
[20]
WANG Z M, GAO H M, FU X T,et al. Multi-components quantitation by one marker new method for quality evaluation of Chinese herbal medicine[J]. China J Chin Mater Med(中国中药杂志),2006,31(23):1925-1928.
[21]
SUN L, JIN H Y, PANG Y,et al. Two reference substances for determination of multiple components (Ⅰ): linear calibration using two reference substances for identification of chromatographic peaks[J]. Chin J Pharm Anal(药物分析杂志),2013,33(8):1424-1430.
[22]
WANG Y Y, SHI X Y, ZHANG Y,et al. Simultaneous quantitative analysis of four triterpenes in Poria Cocos from different habitats by QAMS[J]. Chin Tradit Herb Drugs(中草药),2018,49(20):188-193.
[23]
The United States Pharmacopeial Convention. USP41-NF36 [S]. 2017:4490-4491.
[24]
SUN L, JIN H Y, TIAN R T, et al. A simple method for HPLC retention time prediction: linear calibration using two reference substances[J]. Chin Med, 2017,12(1):1-16.
[25]
SUN L, PANG Y, JIN H Y,et al. Two reference substances for determination of multiple components(Ⅱ):influence of detection wavelength selection on quantitative analysis[J]. Chin J Pharm Anal(药物分析杂志), 2013,33(9):1578-1586.
[26]
PANG Y, SUN L, JIN H Y,et al. Discussion on application and technical requirements of substitute reference substance method for simultaneous determination of multi-components in traditional Chinese medicine[J]. Chin J Pharm Anal(药物分析杂志),2013,33(1):169-177.
2019, Vol. 54 
