Quality Evaluation of Tall Gastrodia Capsules by Simultaneous Determination of the Contents of Multi-Constituents
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1.Department of Pharmacy， The Affiliated Hospital of Guiyang Medical Colledge， Guiyang 550004， China； 2. School of Pharmacy， Guiyang Medical Colledge， Guiyang 550004，China)
ABSTRACT： OBJECTIVE To establish a method for simultaneous determination of the contents of muilti-constituents in tall gastrodia capsules for quality control. METHODS The HPLC was performed on a Phenomenex Hydro-RP 80A (4.6 mm×150 mm， 4 μm) column with a mixture of methanol-0.1% H3PO4 solution as the mobile phase in a linear gradient elution mode. The flow rate was 1 mL·min-1， and the column temperature was set at 40 ℃. The detection wave length was set at 270 nm. RESULTS The linear ranges were 0.40-6.4 μg (r=0.999 7) for gastrodin ， 0.064-1.0 μg(r =0.999 8) for 4-hydroxybenzyl alcohol， 0.020-0.32μg(r =0.999 7) for 4-hydroxybenzaldehyde ， 0.60-9.5 μg (r=0.999 9) for parishin， respectively. The average recovery were 100.8%（RSD 2.3%
OBJECTIVE To establish a method for simultaneous determination of the contents of muilti-constituents in tall gastrodia capsules for quality control. METHODS The HPLC was performed on a Phenomenex Hydro-RP 80A (4.6 mm×150 mm， 4 μm) column with a mixture of methanol-0.1% H3PO4 solution as the mobile phase in a linear gradient elution mode. The flow rate was 1 mL·min-1， and the column temperature was set at 40 ℃. The detection wave length was set at 270 nm. RESULTS The linear ranges were 0.40-6.4 μg (r=0.999 7) for gastrodin ， 0.064-1.0 μg(r =0.999 8) for 4-hydroxybenzyl alcohol， 0.020-0.32μg(r =0.999 7) for 4-hydroxybenzaldehyde ， 0.60-9.5 μg (r=0.999 9) for parishin， respectively. The average recovery were 100.8%（RSD 2.3%）、100.5%（RSD 3.4%）、101.9%（RSD 2.1%）、100.4%（RSD 2.5%）， respectively. Six constituents were determined. The average content of the 6 constituents of 25 samples were 2.189%（RSD 3.40%）. CONCLUSION The method is reliable and can be used for the quality evaluation of tall gastrodia capsules.
Quality Evaluation of Tall Gastrodia Capsules by Simultaneous Determination of the Contents of Multi-Constituents[J]. Chinese Pharmaceutical Journal, 2012, 47(5): 380-383
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参考文献
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