Simultaneous Determination of Phenolic Glycosides in Curculigins Rhizoma by HPLC
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1. Department of Pharmacy， Fujian University of Traditional Chinese Medicine， Fuzhou 350108， China； 2. Department of Pharmacy， School of Pharmacy， Second Military Medical University， Shanghai 200433， China； 3.Department of Graduate Management， Second Military Medical University， Shanghai 200433， China
OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of phenolic glycosides in Curculigins Rhizoma. METHODS Angilent 1100 HPLC on a ZORBAX SB C18 column(4.6 mm×250 mm， 5 μm) with Extend C18 guard column(4.6 mm×12.5 mm， 5 μm) were used. The mobile phase consisted of acetonitrile(A)-0.1% phosphoric acid aqueous(B). The gradient elution program was as follows： 0-25 min， 4%-6%A； 25-58 min， 6%-17%A； 58-85 min， 17%-22%A. The flow rate was kept at 1 mL·min-1， and the column temperature was set at 30 ℃. The detection wavelength was 230 nm. RESULTS The concentrations and peak areas of the 8 phenolic glycosides including 5-hydroxymethylfurfural(1)， 2-hydroxy-5-(2-hydroxyethyl)phenyl-β-D-glucopyranoside(2)， anacardoside(3)， orcinol glucoside(4)， orcinol-1-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside(5)， 2，6-dimethoxybenzoic acid(6)， curculigoside(7) and curculigine A(8) were in good linear relationship. The average recoveries of the 8 constituents met the requirement for determination. CONCLUSION The method is simple， sensitive， reproductive， and suitable for simultaneous determination of a variety of phenolic glycosides in Curculigins Rhizoma.
Simultaneous Determination of Phenolic Glycosides in Curculigins Rhizoma by HPLC[J]. Chinese Pharmaceutical Journal, 2012, 47(5): 375-379
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