摘要
目的 探讨灯盏花素对Aβ42诱导阿尔茨海默病(AD)大鼠模型学习记忆功能及抗氧化能力的影响。方法 采用侧脑室注射Aβ42 的方法制备大鼠痴呆模型， 将其随机为5组，并设假手术组作为对照组，水迷宫实验测试大鼠学习记忆能力的变化；采用分光光度法测定脑组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果 Morris水迷宫实验显示，模型组大鼠逃避潜伏期较正常组明显延长，原象限停留时间明显缩短。灯盏花素组大鼠逃避潜伏期较模型组明显缩短，原象限停留时间明显延长。模型组大鼠脑组织GSH-Px、SOD活力下降，MDA含量明显增多；灯盏花素治疗组学习和记忆能力升高，脑组织GSH-Px、SOD活力升高，MDA含量减少，与模型组比较有显著性差异。结论 灯盏花素对AD模型大鼠学习记忆障碍具有明显改善作用并且可剂量依赖性的显著改善AD模型大鼠的抗氧化能力。
Abstract
OBJECTIVE To study the effect of breviscapine on memory and antioxidation ability in Aβ42-induced Alzheimer’s disease (AD) rat model. METHODS AD rat model was established by intracerebroventricular injection of Aβ42. Besides， sham-operated mice served as control group. The learning and memory ability was determined by Morris water maze test. The activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) and the content of Malondialdehyde (MDA) in brain tissue were measured with spectrophotometer. RESULTS Compared with control group， AD model significantly prolonged the latency of searching the hidden platform in directional swimming test， and decreased time spent in swimming in the target quadrant in probe test. Breviscapine dose-dependently shortened the latency of searching the hidden platform in directional swimming test， and increased the time spent swimming in the target quadrant in probe test. The activities of GSH-Px and SOD were decreased， and the MDA content was increased in AD model rats.In breviscapine treatment groups， the activities of GSH-Px and SOD heightened and MDA content decreased， and there was significant difference compared with AD model group. CONCLUSION Breviscapine can dose-dependently ameliorate the impaired learning and memory function of AD model rats and improve antioxidation ability.
关键词
灯盏花素 /
阿尔茨海默病 /
学习记忆 /
氧化应激
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Key words
breviscapine /
Alzheimer’s disease /
learning and memory /
oxidative stress
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梅峥嵘 司徒冰 黄汉辉 严鹏科 .
灯盏花素对阿尔茨海默病模型大鼠学习记忆和抗氧化能力的影响[J]. 中国药学杂志, 2012, 47(5): 347-350
Influence of Breviscapine on Memory and Antioxidation Ability in Rats with Alzheimer′s Disease[J]. Chinese Pharmaceutical Journal, 2012, 47(5): 347-350
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