Determination of Main Alkaloids in Fritillaria taipaiensis by HPLC-ELSD
DUAN Bao-zhong1， WANG Li-zhi1， HUANG Lin-fang1*， WEI Da-hua2， LU Qi-fu2
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1.Institute of Medicinal Plant Development， Chinese Academy of Medical Sciences & Peking Union Medical College， Beijing 100193，China; 2.Guangzhou Pangaoshou Pharmaceutical Co.， Ltd， Guangzhou 511490， China
OBJECTIVE To develop a HPLC method for determining the contents of peimisine， imperialine and peiminine in Fritillaria taipaiensis. METHODS The analyses were performed on an Agilent Extend-C18 column（4.6 mm×250 mm， 5 μm. The investigated compounds were separated with gradient mobile phase consisting of methanol-water-triethylamine at the flow of 1.0 mL·min-1. The temperature for the detector drift tube was set at 90 ℃ and the nitrogen flow-rate was 2.3 L·min-1， the gain in ELSD was set at 2 in order to obtain the best sensitivity， and the injection volume was 10 μL. RESULTS The investigated compounds including peimisine， peiminine and peimine had good linearity (r2≥0. 9991 within the tested ranges. The average recoveries were 99.4％，99.7％ and 100.3％ with RSD≤3.61%. CONCLUSION The developed method can be applied to control the quality of the F. taipaiensis with a good accuracy， sensitivity and repeatability.
DUN o-zhong;WNG Li-zhi;HUNG Lin-fng;WEI D-hu;LU Qi-fu.
Determination of Main Alkaloids in Fritillaria taipaiensis by HPLC-ELSD[J]. Chinese Pharmaceutical Journal, 2011, 46(5): 382-384
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