摘要
目的 利用高效液相色谱法测定太白贝母中的生物碱成分。方法 采用Agilent Extend C18柱（4.6 mm×250 mm，5 μm，流动相为甲醇-0.02%三乙胺水溶液，梯度洗脱，流速为1.0 mL·min-1，漂移管温度90 ℃；载气体积流量2.3 L·min-1，增益值2，进样量10 μL；柱温25 ℃。结果 贝母辛在28.75～690.0 μg·mL-1，贝母素乙在27.37～560.6 μg·mL-1，贝母素甲在25.75～527.0 μg·mL-1与峰面积的对数值呈线性关系，平均回收率分别为99.4％（n=6，RSD为1.10％；99.7％（n=6，RSD为3.61％；100.3％（n=6，RSD为2.82%。结论 太白贝母中的生物碱成分与川贝母非常相似，为其作为川贝母来源的合理性奠定了基础。
Abstract
OBJECTIVE To develop a HPLC method for determining the contents of peimisine， imperialine and peiminine in Fritillaria taipaiensis. METHODS The analyses were performed on an Agilent Extend-C18 column（4.6 mm×250 mm， 5 μm. The investigated compounds were separated with gradient mobile phase consisting of methanol-water-triethylamine at the flow of 1.0 mL·min-1. The temperature for the detector drift tube was set at 90 ℃ and the nitrogen flow-rate was 2.3 L·min-1， the gain in ELSD was set at 2 in order to obtain the best sensitivity， and the injection volume was 10 μL. RESULTS The investigated compounds including peimisine， peiminine and peimine had good linearity (r2≥0. 9991 within the tested ranges. The average recoveries were 99.4％，99.7％ and 100.3％ with RSD≤3.61%. CONCLUSION The developed method can be applied to control the quality of the F. taipaiensis with a good accuracy， sensitivity and repeatability.
关键词
太白贝母 /
生物碱 /
高效液相色谱-蒸发光检测法
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Key words
Fritillaria taipaiensis /
alkaloids /
HPLC-ELSD
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段宝忠 王丽芝 黄林芳 魏大华 卢其福 .
川贝母新资源太白贝母中生物碱类成分含量测定[J]. 中国药学杂志, 2011, 46(5): 382-384
DUN o-zhong;WNG Li-zhi;HUNG Lin-fng;WEI D-hu;LU Qi-fu.
Determination of Main Alkaloids in Fritillaria taipaiensis by HPLC-ELSD[J]. Chinese Pharmaceutical Journal, 2011, 46(5): 382-384
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参考文献
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