目的 研究以HPLC同时测定肾复舒颗粒（大黄、甘草和枳壳等）中大黄酚、甘草苷和柚皮苷含量的方法。方法 采用Agilent TC-C₁₈柱（4.6 mm×250 mm，5 μm），以甲醇-0.1%冰醋酸为流动相，梯度洗脱0~20 min，甲醇由40%~60%，0.1%冰醋酸由60%~40%；20~40 min，甲醇由60%~85%，0.1%冰醋酸由40%~15%；40~45 min，甲醇85%，0.1%冰醋酸15%，流速：1.0 mL·min-1，λ₁=276 nm（检甘草苷），λ₂=283 nm（检柚皮苷），λ₃=254 nm（检大黄酚），柱温：30 ℃。结果 大黄酚在6.82~136.4 μg·mL-1与峰面积呈良好的线性关系，r=1.000 0，柚皮苷在5.52~82.8 μg·mL-1与峰面积呈良好的线性关系，r=0.999 9，甘草苷在17.52~175.2 μg·mL-1与峰面积呈良好的线性关系，r=1.000 0。平均加样回收率（n=9）：大黄酚为99.9%，柚皮苷为99.6%，甘草苷为100.6%。结论 本法操作简便，结果可靠，重现性好，可作为该产品质量控制的方法。

OBJECTIVE Agilent TC-C₁₈ Column （4. 6 mm×250 mm，5 μm） was used in the determination of chrysophanol，liquiritin and naringin in Shenfushu Granules（Rheum offcinale Baillon，Radix Glycyrrhizae and fructus aurantii） by HPLC-DAD.METHODS The mobile phase was methanol-0.1% glacial acetic acid for gradient elution（0-20 min，methanol from 40% to 60%，0.1% glacial acetic acid from 60% to 40%；20-40 min，methanol from 60% to 85%，0.1% glacial acetic acid from 40% to 15%；40-45 min，methanol 85%，0.1% glacial acetic acid 15%）. The flow rate was 1.0 mL·min-1，λ₁=276 nm（detect the liquiritin），λ₂=283 nm （detect the naringin），λ₃=254 nm（detect the chrysophanol）. The column temperature was at 30 ℃. RESULTS The linear range of chrysophanol was within 6.82-136.4 μg·mL-1，r=1，the recovery was 99. 9% （n=9）. The linear range of naringin was within 5.52-82.8 μg·mL-1，r=0.999 9，the recovery was 99.6% （n=9）. The linear range of liquiritin was within 17.52-175.2 μg·mL-1，r=1.000 0， the recovery was 100.6% （n=9）. CONCLUSION This method has good reproducibility. It can be used for quality control in the production of Shenfushu Granules.