Institute of Chinese Materia Medica，Shanghai University of Traditional Chinese Medicine，a. Ministry of Education Key Laboratory for Standardization of Chinese Medicines，b. Shanghai R&D Center for Standardization of Chinese Medicines，c. The State Administration of Traditional Chinese Medicine Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines，d. The Shanghai Key Laboratory for Compound Chinese medicines，Shanghai 201210， China
OBJECTIVE To develope a HPLC method for quantitative determination of eleutheroside B and eleutheroside E in Acanthopanax senticosus. METHODS The separation of eleutheroside B and eleutheroside E was performed on an Agilent Zorbax SB-C18 column （4.6 mm ×250 mm，5 μm） by gradient elution with 0.5% aqueous phosphoric acid and acetonitrile as the mobile phase. The flow rate was 1.0 mL·min-1 at 25 ℃， and the detection wavelength was set at 220 nm. RESULTS The linear ranges of eleutheroside B and eleutheroside E were 0.35-34.83 and 0.69-69.20 μg·mL-1，respectively，and the correlation coefficient were 0.999 9 and 1.000 0， respectively. The variations for intra- and inter-day precision were less than 3.1% and 3.4%，and the accuracy（ n=9） for eleutheroside B and eleutheroside E were 97.4% （RSD=5.5% ） and 102.7% （RSD=4.3%），respectively. CONCLUSION This method is simple，accurate，reliable and reproducible for the quality control of Acanthopanax senticosus.
朱燕燕 尉小慧 c d 吴弢 c d 黎万奎 c d 王峥涛 c d.
HPLC-PAD测定刺五加中刺五加苷B与刺五加苷E的含量[J]. 中国药学杂志, 2011, 46(16): 1280-1282
ZHU Yn-yn;WEI Xio-hui;c;d;WU To;c;d;LI Wn-kui;c;d;WNG Zheng-to;c;d .
Determination of Eleutheroside B and Eleutheroside E in Acanthopanax senticosus by HPLC-PAD[J]. Chinese Pharmaceutical Journal, 2011, 46(16): 1280-1282
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