OBJECTIVE To study the stabilization effect of the aqueous solutions of polyoxyethylated nonionic surfactants with different polymerization degree of ethylene oxide and different hydrophobic groups on chloramphenicol. METHODS The stability of chloramphenicol in aqueous solution of TX （polyoxyethylene alkylphenol ether），AEO （polyoxyethylene fatty alcohol ether），Tween （polyoxyethylene sorbitan fatty acid ester） and EL （polyoxyethylene castor oil ether） series were investigated by HPLC in accelerated experiments. The location of the chloramphenicol molecules in the micelles was determined by 1H-NMR spectroscopy. RESULTS The hydrolysis kinetics of chloramphenicol showed first order character in the aqueous solutions of surfactants. The hydrolysis rate constants differed with different structures of hydrophobic group，while kept nearly the same with different polymerization degree of ethylene oxide. The 1H-NMR chemical shift of methylene which composed of the palisade layer of the micelles significantly changed. CONCLUSION The chloramphenicol molecules should be trapped into the palisade layer of the micelles. The polymerization degree of ethylene oxide had slight effect on the stabilization of chloramphenicol while the structure of hydrophobic group had significant effect. Polyoxyethylated nonionic surfactant with double bond，such as tween80 and EL，showed better stabilization effect on chloramphenicol.
ZHNG Li-xi;CHEN Zhi-rong;YIN Hong;YUN Shen-feng.
Stability Study of Chloramphenicol in Polyoxyethylated Nonionic Surfactant Aqueous Solution[J]. Chinese Pharmaceutical Journal, 2011, 46(16): 1258-1261
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