目的 建立二巯丁二酸中有关物质的HPLC测定方法。方法 色谱柱为Ultimate XB-C₁₈（4.6 mm×150 mm，5 μm）；流动相A：硫酸氢四丁基铵磷酸盐溶液（含硫酸氢四丁基铵3.2 g·L-1、磷酸二氢钠6.5 g·L-1和乙二胺四乙酸二钠0.09 g·L-1）-甲醇（85∶15），流动相B：甲醇；流速：1.0 mL·min-1；检测波长220 nm；线性梯度洗脱：0 min，100%A；10 min，100%A；20 min，80%A；50 min，80%A。结果 二巯丁二酸与丁炔二酸、各杂质峰之间分离良好。二巯丁二酸在14.0~112.0 μg·mL-1内线性良好，r=0.999 3，检测限为1.8 μg·mL-1；丁炔二酸在10.3~82.3 μg·mL-1内线性良好，r=1.000 0，检测限为0.4 μg·mL-1，丁炔二钠的平均加样回收率为98.3%。结论 本方法专属性强、准确度高，适合测定二巯丁二酸的有关物质。

OBJECTIVE To establish an HPLC method for determination of related substances in dimercaptosuccinic acid. METHODS An Ultimate XB-C₁₈（4.6 mm×150 mm，5 μm） column was applied. Mobile phase A was tetrabutylammonium bisulfate phosphate solution containing tetrabutylammonium bisulfate 3.2 g·L-1， potassium dihydrogen phosphate 6.5 g·L-1 and disodium edenate 0.09 g·L-1， mobile phase B was meathanol. Gradient elution program： 0 min，100%A；10 min，100%A；20 min，80%A；50 min，80%A. The flow rate was 1.0 mL·min-1. Detection wavelength was set at 220 nm. RESULTS The linear range of dimercaptosuccinic acid was 14.0-112.0 μg·mL-1 （r=0.999 3），limit of detection was 1.8 μg·mL-1. The linear range of acetylenedicarboxylic acid was 10.3-82.3 μg·mL-1 （r=1.000 0），limit of detection was 0.4 μg·mL-1，while the average recovery was 98.3%. CONCLUSION This method proved to be specific，accurate and suitable for determining the related substances in dimercaptosuccinic acid.