目的 研究枳壳药材的HPLC指纹图谱及测定其柚皮苷、新橙皮苷和辛弗林3种成分的含量，以建立药材有效的质量评价方法。方法 通过建立HPLC指纹图谱和有效成分含量测定方法，对来自10个产地的枳壳药材进行分析。色谱条件为Alltima C₁₈色谱柱(4.6 mm×250 mm， 5 μm；流动相为乙腈-0.1％十二烷基磺酸钠和0.1％磷酸水溶液，梯度洗脱。流速为1.0 mL·min-1；指纹图谱检测波长为 224 nm，柚皮苷和新橙皮苷含量的检测波长为283 nm，辛弗林含量的检测波长为224 nm。结果 得到分离度、重现性均较好的枳壳药材HPLC指纹图谱，标示了6个共有色谱峰，对10批来自不同产地的枳壳的色谱指纹图谱进行了相似度评价；应用HPLC-DAD测定了10种枳壳样品中柚皮苷、新橙皮苷和辛弗林的含量，结果显示，不同产地枳壳药材中柚皮苷、新橙皮苷和辛弗林含量差异较大。结论 该方法简便准确，灵敏度高、重复性好，可以作为枳壳药材的质量控制方法。不同产地的枳壳药材有效成分(柚皮苷、新橙皮苷和辛弗林含量具有明显差异。

OBJECTIVE To establish the HPLC fingerprints for assessing the quality of Fructus Aurantii from different habitats，and to determine the contents of naringin， neohesperidin and synephrine. METHODS HPLC fingerprinting and content determination methods were applied to evaluate 10 batches of Fructus Aurantii from different habitats.The samples were separated by Alltima C₁₈ column(4.6 mm×250 mm，5 μm using acetonitrile-0.1％ phosphoric acid and 0.1％ SDS water solution as mobile phase with gradient elution.The flow rate was 1.0 mL·min-1. The detection wavelengths were 224 nm for fingerprinting， 283 nm for content determination of naringin and neohesperidin， and 224 nm for content determination of synephrine， respectively.RESULTS HPLC fingerprint of Fructus Aurantii was established with good separation and repeatability，which could be used for quality assessment of Fructus Aurantii.Six common peaks were defined in characteristic fingerprints， and similarity evaluation system was applied to evaluate the fingerprints of the 10 batches of Fructus Aurantii. The contents of naringin， neohesperidin and synephrine in Fructus Aurantii from different habitats were obviously different from each other. CONCLUSION The method is sensitive， repeatable and accurate， which can be used for the quality control of Fructus Aurantii.The contents of naringin， neohesperidin and synephrine vary significantly in Fructus Aurantii from different habitats， which suggests that more attention should be paid to the clinical use of Fructus Aurantii.