显微共聚焦拉曼光谱法检测非法添加的化学壮阳药

王玉 曹玲 罗疆南

中国药学杂志 ›› 2011, Vol. 46 ›› Issue (10) : 789-793.

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中国药学杂志
PDF(1054 KB)
中国药学杂志 ›› 2011, Vol. 46 ›› Issue (10) : 789-793.
论著

显微共聚焦拉曼光谱法检测非法添加的化学壮阳药

  • 王玉1，2，曹玲1*，罗疆南2
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Identification of Adulterated PDE5 Inhibitors by Confocal Raman Microscopy

  • WANG Yu1，2， CAO Ling1*，LUO Jiang-nan2
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文章历史 +

摘要

目的 建立显微共聚焦拉曼光谱法检测中药中非法添加磷酸二酯酶5型（PDE5抑制剂的方法。方法 供试品在显微镜下微区成像，逐点选择不同的点采集拉曼光谱图，并与对照品光谱图进行比对分析，以判断是否非法添加。结果 在市场监督抽样的不同种类的壮阳类中药中分别检出非法添加PDE5抑制剂西地那非及其同系物他达那非，检出结果进一步经HPLC-MS分析验证。结论 显微共聚焦拉曼光谱可作为中药、保健食品中非法添加化学药物检测的可选方法。

Abstract

OBJECTIVE To develop a Raman spectroscopy method for the identification of PDE5 inhibitors adulterated in traditional Chinese medicines. METHODS The spectra of the samples， collected by Confocal Raman microscopy with the spatial mapping and multivariate analysis of Raman maps， were compared with those obtained with the reference substances. RESULTS The PDE5 inhibitors such as Sildenafil and Tadalafil adulterated in the products supplied for inspection were rapidly detected. The results were further verified by HPLC-MS analysis. CONCLUSION The Confocal Raman microscopy can be used as an alternative method for the identification of the adulteration of traditional Chinese medicines and health-care food with synthetic drugs.

关键词

拉曼光谱法 / 显微共聚焦拉曼 / 壮阳类中药 / PDE5抑制剂

Key words

Raman spectroscopy / Confocal Raman microscopy / Chinese traditional medicines / phosphodiesterase-5 inhibitors

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王玉 曹玲 罗疆南. 显微共聚焦拉曼光谱法检测非法添加的化学壮阳药[J]. 中国药学杂志, 2011, 46(10): 789-793
WNG Yu;CO Ling;LUO Jing-nn. Identification of Adulterated PDE5 Inhibitors by Confocal Raman Microscopy[J]. Chinese Pharmaceutical Journal, 2011, 46(10): 789-793

参考文献


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[6] SFDA. Inspection and Additional Testing Methods and Project Approval Documents[S]. Application Number 2006009， Identification of sildenafil and Tadalafil by HPLC/MS/MS, 2006：1-2.
[7] SFDA. Inspection and Additional Testing Methods and Project Approval Documents[S]. Application Number 2009030, Rapid identification of PDE5 inhibitors adulterated in traditional Chinese Medicinal for invigorant, 2009：1-6.
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