目的 建立高效液相色谱法测定七味广枣丸中丁香酚、木香烃内酯和去氢木香内酯含量的方法 。 方法 色谱柱为Agilent ZORBAX Eclipse SB- C₁₈ （4.6 mm×150 mm，5 μm ；流动相为5%乙腈-乙腈（58∶42；流速为 1 mL·min^-1；检测波长程序：0～15.0 min为280 nm；15.1～40 min为225 nm。 结果 丁香酚， 木香烃内酯和去氢木香内酯的保留时间分别约为6， 21和24 min，与相邻峰的分离度大于1.5。以峰面积对进样浓度 （μg·mL^-1 线性回归，丁香酚回归方程为Y=0.104 5ρ-6.161，r=0.999 9， 线性范围 0.552 0～13.80 μg；木香烃内酯回归方程为Y=0.095 39ρ-0.297 2，r=0.999 9，线性范围0.074 00～1.850 μg；去氢木香内酯回归方程为Y=0.078 51ρ-0.079 49，r=0.999 8，线性范围0.059 50～1.488 μg；丁香酚，木香烃内酯和去氢木香内酯的回收率分别为99.2%， 101.3% 和 97.0%，RSD分别为0.89%、1.6%和1.6%。 结论 本方法 操作简便，测定结果 准确，重复性好，可用于七味广枣丸中丁香酚、木香烃内酯和去氢木香内酯含量的同时测定。

OBJECTIVE To establish a HPLC quantitative method for the determination of eugenol， costunolide and dehydrocostuslactone in Qiwei Guangzao pills. METHODS The separation was performed on a C₁₈ column（Agilent ZORBAX Eclipse SB-C₁₈，4.6 mm× 150 mm，5 μm， with the mobile phase consisted of 5% acetonitrile-acetonitrile (58∶42. The flow rate of 1 mL·min^-1. The detective wavelength was set at 280 nm (0～15 min，225 nm (15.1-40 min. RESULTS The retention times of eugenol， costunolide and dehydrocostuslactone were about 6， 21 and 24 min. The resolution was above 1.5. The regress equation for eugenol was Y=0.104 5ρ-6.161，r=0.999 9，and the linear range was 0.552 0-13.80 μg. The equation of costunolide was Y=0.095 39ρ-0.297 2， r=0.999 9，and the linear range was 0.074 00-1.850 μg·mL^-1. The equation of dehydrocostuslactone was Y=0.078 51ρ-0.079 49， r=0.999 8，and the linear range was 0.059 50-1.488 μg. The average recoveries of eugenol， costunolide and dehydrocostuslactone were 99.2%， 101.3% and 97.0% with RSDs of 0.89%， 1.6% and 1.6%. CONCLUSION This method is simple，time-saving and accurate. It can be used for routine analysis of eugenol， costunolide and dehydrocostuslactone in Qiwei Guangzao pills.