目的 建立柴黄片制剂中柴胡皂苷b₁、b₂的HPLC含量测定方法 。 方法 采用Hypersil C₁₈色谱柱（4.6 mm×250 mm，5 μm；流动相：甲醇-水（75∶25；流速：1.0 mL·min^-1；检测波长：254 nm；柱温：30 ℃。 结果 柴胡皂苷b₁的线性范围为0.015～0.120 μg（r=0.999 5，柴胡皂苷b₂的线性范围为0.057～0.456 μg（r=0.999 8，柴胡皂苷b₁、b₂的平均加样回收率分别为97.2%、96.9%，RSD分别为2.0%、1.6%。结论 本方法 简便、可靠、重现性好，可用于柴黄片中柴胡皂苷b₁、b₂的含量测定，为完善含柴胡类成方制剂的质量评价体系提供参考。

OBJECTIVE To establish a HPLC for the quantitative determination of saikosaponin b₁ and b₂ in Chai-huang Tablet. METHODS The HPLC method was performed on a Hypersil C₁₈ column (4.6 mm×250 mm, 5 μm with MeOH - Water (75∶25 as the mobile phase. The flow rate was 1.0 mL·min^-1. The UV detection wavelength was 254 nm， and the column temperature was set at 30 ℃. RESULTS The calibration curves showed good linear response ranged from 0.015 to 0.120 μg (r=0.999 5 for saikosaponin b₁ and from 0.057 to 0.456 μg (r=0.999 8 for saikosaponin b₂. The average recoveries were 97.2% (RSD=2.0% and 96.9% (RSD=1.6% respectively. CONCLUSION The method is simple, reliable and repeatable, and can be used for the quantitative determination of saikosaponin b₁ and b₂ in Chai-huang Tablet.