Experimental Study on Oxymatrine Inducing Apoptosis of Human Carcinoma Cells MGC-803 in Vivo and in Vitro
ZHU Yan-qin1，LIU Fen-xia2，XU Yu-fang1
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1.College of Basic Medical Sciences, Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China； 2. Second Affiliated Hospital，Henan College of Traditional Chinese Medicine，Zhengzhou 450008，China
OBJECTIVE To explore effect of oxymatrine on MGC-803 cell apoptosis and its possible mechanism in vivo and in vitro. METHODS Apoptosis rate and the expression level of Bcl-2 and Bax were determined with flow cytometry. Subcutaneous transplantation tumor cell apoptosis was observed in nude mouse with TUNEL. RESULTS The study in vivo and in vitro indicated that oxymatrine promoted MGC-803 cell apoptosis. The index of apoptosis was significantly different (P﹤0.05 compared with the control group. The expression of Bcl-2 was reduced and the expression of Bax was increased remarkably ( P﹤0.05. CONCLUSION Oxymatrine can induce MGC-803 cell apoptosis in vivo and in vitro. The mechanism was related to up-regulating expression of Bax and down-regulating expression of Bcl-2.
ZHU Yn-qin;LIU Fen-xi;XU Yu-fng.
Experimental Study on Oxymatrine Inducing Apoptosis of Human Carcinoma Cells MGC-803 in Vivo and in Vitro[J]. Chinese Pharmaceutical Journal, 2011, 46(1): 28-30
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