用生物测定法研究肉苁蓉多糖在体外的吸收转运

齐云 蔡润兰 王翔岩 李蒙 李晓红 杨美华

中国药学杂志 ›› 2010, Vol. 45 ›› Issue (7) : 527-530.

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PDF(878 KB)
中国药学杂志 ›› 2010, Vol. 45 ›› Issue (7) : 527-530.
论著

用生物测定法研究肉苁蓉多糖在体外的吸收转运

  • 齐云,蔡润兰,王翔岩,李蒙,李晓红,杨美华
作者信息 +

Absorption and Transportation of Cistanche deserticola Polysaccharides Using Bioactivity Assay in Vitro

  • QI Yun, CAI Run-lan, WANG Xiang-yan, LI Meng, LI Xiao-hong, YANG Mei-hua
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文章历史 +

摘要

目的 用生物测定法考察肉苁蓉多糖(CDPS)在Caco-2细胞模型的吸收转运。方法 用培养于Transwell上的Caco-2细胞模型研究CDPS的吸收转运;采用生物活性测定法测定CDPS在接受侧介质中的含量。结果 Caco-2细胞单层极性与完整性符合实验要求,CDPS在1.87 ~ 40 mg·L-1内,与RAW264.7细胞NO分泌呈明显量效关系,转运量随供侧(AP侧)浓度的增加而增大,不同浓度CDPS Papp( A-B)均小于1 × 10-7 cm·s-1。结论 采用生物活性测定CDPS的Papp具可行性,该药属吸收不良药物,口服吸收率应小于1%。

关键词

肉苁蓉多糖 / Caco-2细胞单层模型

Key words

Cistanche deserticola polysaccharides (CDPS) / Caco-2 cell monolayer model / apparent permeability coefficient (Papp) / absorption transport

引用本文

导出引用
齐云 蔡润兰 王翔岩 李蒙 李晓红 杨美华. 用生物测定法研究肉苁蓉多糖在体外的吸收转运[J]. 中国药学杂志, 2010, 45(7): 527-530
QI Yun;CI Run-ln;WNG Xing-yn;LI Meng;LI Xio-hong;YNG Mei-hu. Absorption and Transportation of Cistanche deserticola Polysaccharides Using Bioactivity Assay in Vitro[J]. Chinese Pharmaceutical Journal, 2010, 45(7): 527-530

参考文献


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