摘要
目的 比较α-双炔失碳酯（α-Ano）对激素依赖型与非激素依赖型前列腺癌体内外作用强弱的差别。方法 应用SRB细胞染色法检测α-Ano对人前列腺癌激素依赖型细胞LNCaP、RV1、L1A和非激素依赖细胞DU145、PC-3生长的影响；裸鼠皮下接种人前列腺癌细胞RV1、PC-3，构建实体瘤模型，成瘤后分组并灌胃给药，观察测量肿瘤体积及质量变化，计算肿瘤增长百分率和抑瘤率，比较α-Ano对激素依赖型和非激素依赖型人前列腺癌裸鼠皮下移植瘤生长的抑制作用。结果 α-Ano作用后，LNCaP、RV1和L1A细胞生存率均低于DU145、PC-3细胞；α-Ano高低剂量组均对裸鼠皮下RV1细胞移植瘤具有较强的抑制生长作用，而对PC-3细胞移植瘤作用较弱，仅高剂量组有一定抑制作用。 结论 α-Ano对激素依赖型前列腺癌的抑制作用强于非激素依赖型前列腺癌。
Abstract
OBJECTIVE To observe the effects of Earthworm injection on the human bronchial epithelial cells（HBE） sensitized by house dust mite allergen Dermatophagoides pteronyssinus and human embryonic lung fibroblast(HLF)， and to investigate the mechanism on the treatment of asthma. METHODS Using different intervention and treatment，HBE were divided into 6 groups， control group（group A）， Derp1 allergen（10 μg·mL-1）group（group B）， Derp1 allergen（10 μg·mL-1）+ Dexamethasone(DEX)（1×10-6 mol·L-1） group (group C)， Derp1 allergen（10 μg·mL-1）+ Earthworm iniection（0.9 mg·mL-1）group（group D）， Derp1 allergen（10 μg·mL-1）+ Earthworm injection（9 mg·mL-1）group（group E）， Derp1 allergen（10 μg·mL-1）+ Earthworm injection（18 mg·mL-1）group（group F）， cultured for 72 h.Then the expression of transforming growth factors of （TGF）-β1（TGF-β1） mRNA and Smad2 mRNA were determined by RT-PCR. The supernatants of each group were collected and inculoated with the HLF for 72 h. The expression of fibronectin（FN） was detected by immunocytochemical method. RESULTS Compared with the group A， TGF-β1 mRNA and Smad2 mRNA in group B were increased significantly(P<0.01). The TGF-β1 mRNA and Smad2 mRNA in group D， group E， group F and group C were all decreased compared with group B（P<0.01） and no significant difference from group A（P>0.05）. After inculoating with HLF for 72 h. The expression of FN was relative with the change of TGF-β1 mRNA， in positive correlation. CONCLUSION The earthworm injection had an inhibitory action on airway remodeling of asthma．It significantly depressed the signaling pathway of TGF-β1/Smad2 and may be a role in the modulating mechanism of asthma.
关键词
α-双炔失碳酯 /
前列腺癌 /
激素依赖型 /
非激素依赖型
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Key words
α-anordrin /
human prostatic cancer /
androgen-dependence /
androgen-independence /
nude mouse xenografts tumor
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翁秀华 翁绳美.
α-双炔失碳酯抗激素依赖型与非激素依赖型前列腺癌的作用比较[J]. 中国药学杂志, 2010, 45(15): 1149-1153
WENG Xiu-hu;WENG Sheng-mei.
Comparison of the Effect of α-Anordrin on Androgen-Dependent and Independent Human Prostate Cancer[J]. Chinese Pharmaceutical Journal, 2010, 45(15): 1149-1153
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