目的 建立一种检测人绒毛滋养细胞上清培养液中 <> L - 色氨酸 (<>L-tryptophan ， <> L -Trp) 的高效液相色谱 - 紫外检测法。 方法 采用的色谱柱为 Eclipse XDB-C₁₈ 柱 (4.6 mm×150 mm ， 5 μm) ，流动相为 0.05 mmol·L^-1 磷酸二氢钾溶液 - 甲醇（ 92 ∶ 8 ），流速为 1.3 mL·min^-1 。紫外检测波长为 225 nm 。滋养细胞上清培养液标本经 5.0%( 体积分数 ) 高氯酸溶液去除蛋白质后取上清液过滤后直接进样分析测定。 结果 <> L -Trp 保留时间为 7.5 min ，线性范围为 1.0~100.0 μmol·L^-1 ，最低检出浓度为 0.5 μmol·L^-1 ，回收率为 95.9%~102.0% 。日内、日间测定的相对标准偏差均小于 3% 。加入 1- 甲基色氨酸 (1-methyl tryptophan ， 1-MT) 的上清培养液中 <> L -Trp 的浓度高于未加 1-MT 的上清培养液。 结论 该方法简便、快速、稳定、可行，不同条件下 <> L -Trp 的浓度变化可间接证实人绒毛滋养细胞自身存在其他类似吲哚胺 2 ， 3- 二氧化酶（ indoleamine 2 ， 3-dioxygenase ， IDO ）的方式调节 T 淋巴细胞免疫功能。

OBJECTIVE To establish a method for the determination of L-tryptophan(L-Trp) by HPLC-UV. METHODS ■The separation was performed on a Eclipse XDB-C18 column (4.6 mm×150 mm，5 μm) with 0.05 mmol·L-1 potassium dihydrogan phosphate - methanol (92∶8) as the mobile phase. The detection wavelength was 225 nm and the flow rate was 1.3 mL·min-1. The samples of villous cytotrophoblast substrate were first precipitated with 5.0% perchloric acid solution, then centrifuged to remove protein residue and finally analyzed by HPLC. RESULTS The retention time of L-Trp was 7.5 min, the linear range of the method was from 1.0 to 100.0 μmol·L-1, and the detection limit was 0.5 μmol·L-1. The recoveries of L-Trp were from 95.9%~102%, the intraday and interday variations were less than 3%. The concertration of L-Trp in vitro villous cytotrophoblast with 1-MT was more than the samples without 1-MT. CONCLUSION The method is simple, fast, accurate, and suitable for routine analysis. The concentrations of L-Trp at different condition suggest the different mechanism by which trophoblasts inhibit T cells proliferation in vitro.