宜宾地区蒲公英药材 HPLC 特征图谱定性研究

苏英;彭章明;刘燕;陈代宏

中国药学杂志 ›› 2009, Vol. 44 ›› Issue (24) : 1858-1861.

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PDF(2294 KB)
中国药学杂志 ›› 2009, Vol. 44 ›› Issue (24) : 1858-1861.
论文

宜宾地区蒲公英药材 HPLC 特征图谱定性研究

  • 苏英,彭章明,刘燕, 陈代宏
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HPLC Specific Chromatograms of Herba Taraxaci from Different Areas of Yibin

  • SU Ying, PENG Zhang-ming , LIU Yan, CHEN Dai-hong
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摘要

目的 研究宜宾地区蒲公英药材 HPLC 特征图谱实验方法,建立蒲公英药材的 HPLC 特征图谱并运用 HPLC-MS/MS 技术对主要色谱峰进行定性分析。 方法 采用 RP-HPLC ,梯度洗脱测定 10 批样品。色谱条件为: Waters symmetry shieldTMC18 柱( 4.6 mm ×250 mm , 5 μm );流动相为 A 为乙腈、 B 为 0.1% 磷酸, 流速为 1.0 mL·min-1 ; 检测波长为 210 nm 。 结果 10 批不同乡镇样品的特征图谱中共检出 17 个 相对位置稳定的共有峰;通过 HPLC-MS/MS 技术对其中 6 个峰初步鉴定为: 单咖啡酰酒石酸、绿原酸、咖啡酸、木犀草素葡萄糖苷、菊苣酸、木犀草素。各样品特征图谱与对照特征图谱的 相似度均大于 0.82 。 结论 该方法准确、可靠,可为蒲公英药材质量控制提供科学依据。

Abstract

OBJECTIVE To study the HPLC specific chromatograms testing method of Herba Taraxaci from different areas of Yibin to develope. The specific chromatogram mode and to identify the primary chromatogram peaks by HPLC-MS/MS. METHODS HPLC was utilizaed for quality assessment of 10 batches of samples. RP-HPLC analysis was performed on a Waters symmetry shieldTM C18 column (4.6 mm×250 mm, 5 μm)with the mixture of acetonitrile (A) and 0.1% phosphonic acid solution(B) as mobile phase in gradient mode. The flow rate was 1.0 mL·min-1. The detective UV wavelength was 210 nm. RESULTS 17 common peaks from the specific chromatograms of 10 batches of Herba Taraxaci were determined,6 of which were identified by HPLC-MS/MS . The 6 peaks were as follows: caftaric acid, chlorogenic acid, caffeic acid , 1uteolin-O-glucoside, chicoric acid and 1uteolin .The similarities of the specific chromatograms of 10 batches of samples were all above 0.82. CONCLUSION This method is accurate and reliability. It could be used for quality control of Herba Taraxaci.

关键词

蒲公英 / 特征图谱 / 高效液相色谱 - 质谱联用 / 定性分析

Key words

Herba Taraxaci / specific chromatogram / HPLC-MS/MS / qualitative determination

引用本文

导出引用
苏英;彭章明;刘燕;陈代宏 . 宜宾地区蒲公英药材 HPLC 特征图谱定性研究[J]. 中国药学杂志, 2009, 44(24): 1858-1861
SU Ying;PENG Zhng-ming;LIU Yn;CHEN Di-hong . HPLC Specific Chromatograms of Herba Taraxaci from Different Areas of Yibin[J]. Chinese Pharmaceutical Journal, 2009, 44(24): 1858-1861

参考文献


[1] Ch.P(2005) Vol Ⅰ ( 《 中国药典》 2005 年版 . 一部 ) [S]. 2005: 17 .
[2] WU X C , DU S L , CHEN H S . Research and application of taraxacum herb [J] . J Pharm Pract ( 药学实践杂志 ) , 2002 , 20 ( 4 ) : 246-248 .
[3] LIN Y , ZHENG J H . Research progress of a Chinese traditional drug taraxacum herb [J] . Chin J Mod Appl Pharm ( 中国现代应用药学 ) , 1998 , 15 ( 3 ) : 10-13 .
[4] XIU Y, JING H. The pharmacological action of taraxacum herb [J]. World Notes Plant Med (国外医药植物药分册), 2008 , 23 ( 1 ): 11-12.
[5] MARIA W. Polyphenolic compounds of dandelion[J] . Acta Pol Pharm,1985,42(2): 215 .
[6] HUANG C J , LIN X D , LI J , et al . Research progress of chemical compositions of taraxacum herb [J] . Mod Chin Med ( 中国现代中药 ) , 2006 , 8 ( 5 ) : 32-35 .
[7] LIN Y , FAN G Q , XIAO Y , et al . Studies on quality standard of dandelions (taraxacum weber) [J] . Chin Tradit Herb Drugs ( 中草药 ) , 1999 , 30 ( 12 ) : 897-899 .
[8] MA X Y, CUI L L, XUE Z Y, et al. Study on the content of actine ingredients and gathering time of taraxacum herb [J]. J Hebei Tradit Chin Med Pharm (河北中医药学报) , 2009 , 24 ( 1 ): 41-42.
( 收稿日期 : 2009-06-12 )


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