OBJECTIVE To study the HPLC specific chromatograms testing method of Herba Taraxaci from different areas of Yibin to develope. The specific chromatogram mode and to identify the primary chromatogram peaks by HPLC-MS/MS. METHODS HPLC was utilizaed for quality assessment of 10 batches of samples. RP-HPLC analysis was performed on a Waters symmetry shieldTM C18 column (4.6 mm×250 mm, 5 μm)with the mixture of acetonitrile (A) and 0.1% phosphonic acid solution(B) as mobile phase in gradient mode. The flow rate was 1.0 mL·min-1. The detective UV wavelength was 210 nm. RESULTS 17 common peaks from the specific chromatograms of 10 batches of Herba Taraxaci were determined,6 of which were identified by HPLC-MS/MS . The 6 peaks were as follows: caftaric acid, chlorogenic acid, caffeic acid , 1uteolin-O-glucoside, chicoric acid and 1uteolin .The similarities of the specific chromatograms of 10 batches of samples were all above 0.82. CONCLUSION This method is accurate and reliability. It could be used for quality control of Herba Taraxaci.
SU Ying;PENG Zhng-ming;LIU Yn;CHEN Di-hong .
HPLC Specific Chromatograms of Herba Taraxaci from Different Areas of Yibin[J]. Chinese Pharmaceutical Journal, 2009, 44(24): 1858-1861
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