目的 研究美罗培南 （ meropenem ， MEM ）对铜绿假单胞菌 PAO1 野生型 及其 PAO1 <>lasI rhlI 、 PAO1 <>lasR rhlR 基因缺陷型菌株早期生物膜的作用。 方法 ① 测定 美罗培南 对铜绿假单胞菌 PAO1 野生型 及其 PAO1 <>lasI rhlI 和 PAO1 <>lasR rhlR 基因缺陷型菌株的最低抑菌浓度（ MIC ）和最低杀菌浓度（ MBC ）。②采用生理盐水 - 吸痰管系统进行铜绿假单胞菌 PAO1 野生型、 PAO1 <>lasI rhlI 和 PAO1 <>lasR rhlR 基因缺陷型 体外生物膜培养 3 d 后 ， 用 26 m g·L-1 的美罗培南溶液作用 24 h ， 然 后 用扫描电子显微镜观察 生物膜 的情况。 结果 ① PAO1 野生型， PAO1<> lasI rhlI 基因缺陷型和 PAO1<> lasR rhlR 基因缺陷型 3 种铜绿假单胞菌菌株对 MEM 的 MIC 均为 1 m g·L-1 ， MBC 均为 2 m g·L-1 。 ② 3 d 后 PAO1 野生型形成较厚、有孔状通道的生物膜，而 lasI rhlI 和 lasR rhlR 基因缺陷型菌株形成结构较简单的薄片状的早期生物膜结构；美罗培南作用后， PAO1 野生型的生物膜结构明显稀薄，而 PAO1 <>lasI rhlI 、 PAO1 <>lasR rhlR 基因缺陷型的片状生物膜结构基本被破坏，剩下散在的碎片状结构。 结论 铜绿假单胞菌的 <> las 、 <> rhl 基因可影响铜绿假单胞菌早期生物膜的形成及其对美罗培南的敏感性。
Abstract
OBJECTIVE To study the effect of meropenem
<> on the biofilms of
<> P. aeruginosa <>las/rhl mutation
<> in vitro . METHODS The MIC and MBC of meropenem (MEM) were measured in
<>P. aeruginosa PAO1,
lasI rhlI and
lasR rhlR mutants. The biofilms of PAO1,
lasI rhlI and
lasR rhlR mutants were incubated in a po lyvinyl chloride tube in 0.9% saline for three days ， and then immersed in meropenem solution of 26 mg·L
-1 for 24 hours ， followed by examinations of scanning electron i c microscopy (SEM). RESULTS (1) The MIC and MBC of MEM in PAO1,
lasI rhlI and
lasR rhlR mutants were 1 and 2 mg·L
-1 respectively. （ 2 ） After 3 day’s incubation, PAO1 biofilms showed a well-developed structure. However, the biofilms of
lasR rhlR and
<>lasI rhlI mutants were thin and poor developed. After incubation for 24 hours in meropenem solution, PAO1 biofilm became pellicle-shaped ， whereas
<>lasR rhlR and
<>lasI rhlI mutants biofilms were almost destructed and there were only small pieces left. CONCLUSION
<>las/rhl genes play probably an important role in the formation of
<>P.aeruginosa biofilm and the resistance to meropenem.
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关键词
生物膜 /
群体感应 /
铜绿假单胞菌 /
美罗培南
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Key words
biofilms /
quorum sensing /
Pseudomonas aeruginosa /
meropenem
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参考文献
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[8] SMITH R S, HARRIS S G, PHIPPS R, <>et al. The <>Pseudomonas aeruginosa quorum-sensing molecule n-(3-oxododecanoyl) homoserine lactone contributes to virulence and induces inflammation <>in vivo[J]. <>J Bacteriol, 2002, 184(4): 1132-1139.
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（ 收稿日期 ： 2008-12-22 ）
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