目的 建立 RP-HPLC 测定血浆中阿苯达唑及其代谢物阿苯达唑亚砜、阿苯达唑砜浓度的方法。 方法 在改进文献报道的基础上，采用 C 2 柱固相萃取法处理血浆，建立了精确、灵敏、重现性和专一性好的高效液相 - 紫外检测法（ HPLC-UV ）。色谱条件：分析柱： Agilent Zorbax RX-C8 （ 4.6 mm×250 mm ， 5 μm ）；柱温：室温；流动相：乙腈 -NaAc 缓冲液（ 0.1 mol·L-1 ， HAc 调节 pH 5.0 ） =45 ∶ 55 ，流速 0.9 mL·min-1 ，检测波长 291 nm 。 结果 阿苯达唑及其代谢物阿苯达唑亚砜、阿苯达唑砜的血药浓度线性范围分别为 10~600 ， 10~1 000 ， 10~300 μg·L-1 ；方法回收率分别为 92.23% ~ 103.81% ， 99.59% ~ 100.21% ， 96.89% ~ 106.84% 。 批内精密度分别为 2.03%~3.12% ， 2.05%~3.80% ， 2.79%~4.14% ；批间精密度分别为 3.20% ～ 4.33% ， 2.05%~3.48% ， 2.87%~3.87% 。 结论 本法灵敏、准确，可用于阿苯达唑的人体药动学研究。
Abstract
OBJECTIVE To establish a RP-HPLC method for the determination of albendazole (ABZ), albendazole sulphoxide (ABZSO) and albendazole sulphone(ABZSO2) in human plasma. METHODS The drugs was extracted from plasma with C2 column by SPE method. The compounds were separated on an Agilent Zorbax RX-C8 column（4.6 mm×250 mm，5 μm）by Waters 2690 HPLC. The mobile phase consisted of acetonitrile-NaAc buffer (0.1 mol·L-1, adjusted to pH 5.0 with acetic acid). The detection wavelength was 291 nm. The flow rate was 1.0 mL·min-1. RESULTS A good linearity was obtained over the range of 10~600 μg·L-1 for ABZ, 10~1 000 μg·L-1 for ABZSO and 10~300 μg·L-1 for ABZSO2. The recoveries of method were from 92.23%~103.81% for ABZ、99.59%~100.21% for ABZSO, 96.89%~106.84% for ABZSO2. The precisions of within-run were 2.03%~3.12%, 2.05%~3.80% and 2.79%~4.14%, respectively, and the precisions of between-run were 3.20%~4.33%, 2.05%~3.48% and 2.87%~3.87%, respectively. CONCLUSION The method is sensitive, accurate and reliable, and can be used in clinical research of ABZ.
关键词
阿苯达唑 /
阿苯达唑亚砜 /
阿苯达唑砜 /
血药浓度 /
高效液相色谱法
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Key words
albendazole /
albendazole sulphoxide /
albendazole sulphone /
plasma drug level
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参考文献
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[4] PROCHAZKOVA A, CHOUKI M, THEURILLAT R, <>et al. Therapeutic drug monitoring of albendazole: Determination of albendazole, albendazole sulfoxide, and albendazole sulfone in human plasma using nonaqueous capillary electrophoresis [J]. <>Electrophoresis, 2000, 21(4): 729-736.
[5] GUAN T Y, GONG X Y, SUN W Z, <>et al. Pharmacokinetics of albendazole and its metabolites in human body [J]. <>Acta Pharmacol Sin ( 中国药理学报 ), 1990, 11(1): 69-72.
[6] CHEN X Y, ZHAO L Y, XU H Y, <>et al. Simultaneous determination of albendazole and its major active metabolite in human plasma using a sensitive and specific liquid chromatographic-tandem mass spectrometric method [J]. <> J <> Pharm Biomed Anal , 2004, 35(4): 829-836.
（ 收稿日期 ： 2009-03-17 ）
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